論文 - 詳細
RRC ID | 48790 |
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著者 | Oh K, Matsumoto T, Hoshi T, Yoshizawa Y. |
タイトル | In vitro and in vivo evidence for the inhibition of brassinosteroid synthesis by propiconazole through interference with side chain hydroxylation. |
ジャーナル | Plant Signal Behav |
Abstract |
We carried out the biochemical evaluation of the target site of propiconazole in BR biosynthesis. Applying BR biosynthesis intermediates to Arabidopsis seedlings grown in the presence of propiconazole under dark condition, we found that the target site of propiconazole in BR biosynthesis can be identified among the C22 and C23 side chain hydroxylation steps from campestanol to teasterone. Using differential spectra techniques to determine the binding affinity of propiconazole to CYP90D1, which is responsible for C23 hydroxylation of BR, we found that propiconazole induced typical type II binding spectra in response to purified recombinant CYP90D1 and the Kd value was found approximately 0.76 μM. |
巻・号 | 11(5) |
ページ | e1158372 |
公開日 | 2016-5-3 |
DOI | 10.1080/15592324.2016.1158372 |
PMID | 26987039 |
PMC | PMC4977458 |
MeSH | Arabidopsis / drug effects Arabidopsis / enzymology Arabidopsis / metabolism* Arabidopsis Proteins / metabolism Brassinosteroids / biosynthesis* Cytochrome P-450 Enzyme System / metabolism Hydroxylation / drug effects Kinetics Protein Binding / drug effects Recombinant Proteins / metabolism Seedlings / drug effects Seedlings / metabolism Triazoles / chemistry Triazoles / pharmacology* |
IF | 1.671 |
引用数 | 4 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY PLANT SCIENCES |
リソース情報 | |
シロイヌナズナ / 植物培養細胞・遺伝子 | pda13118 |