| RRC ID |
30605
|
| 著者 |
Kato Y, Kusakabe R, Inoue K, Tochinai S.
|
| タイトル |
MiR-124 is involved in post-transcriptional regulation of Polypyrimidine tract binding protein 1 (PTBP1) during neural development in the medaka, Oryzias latipes.
|
| ジャーナル |
Zoolog Sci
|
| Abstract |
MicroRNAs (miRNAs) comprise a group of small noncoding RNA molecules thought to have contributed to the evolution of vertebrate brain homogeneity and diversity. The miRNA miR-124 is well conserved between invertebrates and vertebrates and is expressed abundantly in the central nervous system (CNS). We identified miR-124 in the medaka, Oryzias latipes, and investigated its role in neural development. The five candidate genes for medaka precursor miR-124 are unlinked on four different chromosomes and differ in nucleotide length. Their sequences suggest that they can generate functional miRNAs through conventional miRNA biogenesis by folding into stem-loop structures. Whole-mount in situ hybridization and northern blotting revealed that mature miR-124 is specifically expressed in the CNS and the eyes starting at two days post-fertilization. We also examined the sequences and expression of medaka Polypyrimidine tract binding protein 1 (Ptbp1), a possible direct target of miR-124. The 3'UTR of medaka Ptbp1 contains predicted binding motifs (target sites) for miR-124. A GFP reporter assay for the target sites or the entire 3'UTR showed that exogenous miR-124 silences PTBP1 expression in vivo. Our study suggests that medaka miR-124 is involved in post-transcriptional regulation of target genes in neural development and that medaka miR-124 homologs may have spatiotemporal roles different from those in other vertebrates.
|
| 巻・号 |
30(11)
|
| ページ |
891-900
|
| 公開日 |
2013-11-1
|
| DOI |
10.2108/zsj.30.891
|
| PMID |
24199853
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Embryo, Nonmammalian / metabolism
Embryonic Development / physiology
Gene Expression Regulation, Developmental / physiology*
MicroRNAs / genetics
MicroRNAs / metabolism*
Molecular Sequence Data
Nucleic Acid Conformation
Oryzias / embryology*
Polypyrimidine Tract-Binding Protein / genetics
Polypyrimidine Tract-Binding Protein / metabolism*
RNA Processing, Post-Transcriptional / physiology*
|
| IF |
0.843
|
| 引用数 |
1
|
|
WOS 分野
|
ZOOLOGY
|
| リソース情報 |
| メダカ |
hatching enzyme
cDNA |
