RRC ID 39253
著者 Matsui H, Ohnishi J, Takahashi T.
タイトル Proteolytic activation of tissue-type plasminogen activator by the culture media of mouse cancer cells.
ジャーナル Zoolog Sci
Abstract Human single-chain tissue-type plasminogen activator (tPA) was activated by the culture media of mouse B16 melanoma and Lewis lung carcinoma cells. This activation was due to the proteolytic conversion of single-chain tPA to two-chain tPA. Typical serine proteinase inhibitors, such as diisopropylfluorophosphate and aprotinin, strongly inhibited the proteolytic activation, suggesting that the enyzme responsible for this activation is a serine proteinase. Through a process of gel filtration, the molecular weight of the putative tPA-activating enzyme was estimated to be approximately 35 kDa. Expression of the tPA mRNA was demonstrated by Northern blot analysis both for the melanoma and carcinoma cells. Zymographic experiments showed that the culture medium of the melanoma cells contained active two-chain tPA. These results indicate that a common serine proteinase may be involved in the proteolytic activation of single-chain tPA in these cancer cells.
巻・号 15(4)
ページ 499-505
公開日 1998-8-1
DOI 10.2108/0289-0003(1998)15[499:PAOTPA]2.0.CO;2
PII 0289-0003-15-4-499
PMID 18462028
IF 0.843
引用数 6
WOS 分野 ZOOLOGY
リソース情報
ヒト・動物細胞 B16 melanoma 4A5(RCB0557) LLC(RCB0558)