RRC ID 35711
著者 Shikata M, Hoshikawa K, Ariizumi T, Fukuda N, Yamazaki Y, Ezura H.
タイトル TOMATOMA Update: Phenotypic and Metabolite Information in the Micro-Tom Mutant Resource.
ジャーナル Plant Cell Physiol
Abstract TOMATOMA (http://tomatoma.nbrp.jp/) is a tomato mutant database providing visible phenotypic data of tomato mutant lines generated by ethylmethane sulfonate (EMS) treatment or γ-ray irradiation in the genetic background of Micro-Tom, a small and rapidly growing variety. To increase mutation efficiency further, mutagenized M3 seeds were subjected to a second round of EMS treatment; M3M1 populations were generated. These plants were self-pollinated, and 4,952 lines of M3M2 mutagenized seeds were generated. We checked for visible phenotypes in the M3M2 plants, and 618 mutant lines with 1,194 phenotypic categories were identified. In addition to the phenotypic information, we investigated Brix values and carotenoid contents in the fruits of individual mutants. Of 466 samples from 171 mutant lines, Brix values and carotenoid contents were between 3.2% and 11.6% and 6.9 and 37.3 µg g(-1) FW, respectively. This metabolite information concerning the mutant fruits would be useful in breeding programs as well as for the elucidation of metabolic regulation. Researchers are able to browse and search this phenotypic and metabolite information and order seeds of individual mutants via TOMATOMA. Our new Micro-Tom double-mutagenized populations and the metabolic information could provide a valuable genetic toolkit to accelerate tomato research and potential breeding programs.
巻・号 57(1)
ページ e11
公開日 2016-1-1
DOI 10.1093/pcp/pcv194
PII pcv194
PMID 26719120
MeSH Breeding Databases, Genetic* Ethyl Methanesulfonate Fruit / genetics Fruit / metabolism Mutagenesis Mutation Phenotype Seeds / genetics Seeds / metabolism Solanum lycopersicum / genetics* Solanum lycopersicum / metabolism
IF 4.062
引用数 21
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
トマト TOMJPF00001