RRC ID 51420
著者 Kurup N, Yan D, Kono K, Jin Y.
タイトル Differential regulation of polarized synaptic vesicle trafficking and synapse stability in neural circuit rewiring in Caenorhabditis elegans.
ジャーナル PLoS Genet
Abstract Neural circuits are dynamic, with activity-dependent changes in synapse density and connectivity peaking during different phases of animal development. In C. elegans, young larvae form mature motor circuits through a dramatic switch in GABAergic neuron connectivity, by concomitant elimination of existing synapses and formation of new synapses that are maintained throughout adulthood. We have previously shown that an increase in microtubule dynamics during motor circuit rewiring facilitates new synapse formation. Here, we further investigate cellular control of circuit rewiring through the analysis of mutants obtained in a forward genetic screen. Using live imaging, we characterize novel mutations that alter cargo binding in the dynein motor complex and enhance anterograde synaptic vesicle movement during remodeling, providing in vivo evidence for the tug-of-war between kinesin and dynein in fast axonal transport. We also find that a casein kinase homolog, TTBK-3, inhibits stabilization of nascent synapses in their new locations, a previously unexplored facet of structural plasticity of synapses. Our study delineates temporally distinct signaling pathways that are required for effective neural circuit refinement.
巻・号 13(6)
ページ e1006844
公開日 2017-6-1
DOI 10.1371/journal.pgen.1006844
PII PGENETICS-D-17-00493
PMID 28636662
PMC PMC5500376
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Dyneins / genetics GABAergic Neurons / metabolism Kinesins / genetics Larva / genetics Larva / growth & development Microtubules / genetics Microtubules / metabolism Neuronal Plasticity / genetics Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism Protein Transport / genetics Signal Transduction Synapses / genetics* Synapses / physiology Synaptic Vesicles / genetics* Synaptic Vesicles / metabolism
IF 5.175
引用数 3
リソース情報
線虫 tm4006