RRC ID 50027
著者 Mukai H, Takahashi M, Watanabe Y.
タイトル Potential usefulness of Brevibacillus for bacterial cancer therapy: intratumoral provision of tumor necrosis factor-α and anticancer effects.
ジャーナル Cancer Gene Ther
Abstract Bacterial cancer therapy, wherein bacteria are used as a gene expression system for the exogenous protein of interest in the body, has started becoming a focus area of research; therefore, studying potential bacterial species for use is extremely important. Here, we investigated the use of Brevibacillus choshinensis as an effective and safe provider of anticancer proteins in the body, using a transformant expressing murine tumor necrosis factor-α (mTNF-α). The transformant sustainably provided mTNF-α in tumors in mice for a few hours post-injection. The growth of TNF-α-sensitive tumors was inhibited even by the control transformant, which did not provide mTNF-α; intratumoral mTNF-α provision by Brevibacillus choshinensis had additive effects on tumor growth inhibition. In contrast, intratumorally injected recombinant mTNF-α did not inhibit tumor growth because of rapid elimination from the tumor. Blood biochemical and histochemical analyses showed that intravenous injection of the transformant that did not provide mTNF-α did not lead to tissue injury and dysfunction or infiltration of inflammatory cells over 1 week. Considering the findings, this approach is expected to have a high degree of usability as a delivery system for protein pharmaceuticals, especially from the viewpoints of loading capacity and cost effectiveness.
巻・号 25(3-4)
ページ 47-57
公開日 2018-5-1
DOI 10.1038/s41417-017-0009-7
PII 10.1038/s41417-017-0009-7
PMID 29255215
MeSH Animals Brevibacillus* / genetics Brevibacillus* / immunology Cell Line, Tumor Male Mice Mice, Inbred BALB C Mice, Nude Mice, SCID Neoplasms, Experimental* / genetics Neoplasms, Experimental* / immunology Neoplasms, Experimental* / therapy Tumor Necrosis Factor-alpha* / genetics Tumor Necrosis Factor-alpha* / immunology
IF 4.534
引用数 1
リソース情報
遺伝子材料 pBluescript SKII+mTNF alpha (RDB01478).
ヒト・動物細胞 L929(RCB1422)