RRC ID 49348
著者 Sasaki T, Sato Y, Higashiyama T, Sasaki N.
タイトル Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells.
ジャーナル Sci Rep
Abstract Mitochondrial DNA (mtDNA) is organized in nucleoprotein complexes called mitochondrial nucleoids (mt-nucleoids), which are critical units of mtDNA replication and transmission. In humans, several hundreds of mt-nucleoids exist in a cell. However, how numerous mt-nucleoids are maintained during the cell cycle remains elusive, because cell cycle synchronization procedures affect mtDNA replication. Here, we analyzed regulation of the maintenance of mt-nucleoids in the cell cycle, using a fluorescent cell cycle indicator, Fucci2. Live imaging of mt-nucleoids with higher temporal resolution showed frequent attachment and detachment of mt-nucleoids throughout the cell cycle. TFAM, an mtDNA packaging protein, was involved in the regulation of this dynamic process, which was important for maintaining proper mt-nucleoid number. Both an increase in mt-nucleoid number and activation of mtDNA replication occurred during S phase. To increase mt-nucleoid number, mtDNA replication, but not nuclear DNA replication, was necessary. We propose that these dynamic and regulatory processes in the cell cycle maintain several hundred mt-nucleoids in proliferating cells.
巻・号 7(1)
ページ 11257
公開日 2017-9-12
DOI 10.1038/s41598-017-10843-8
PII 10.1038/s41598-017-10843-8
PMID 28900194
PMC PMC5595809
MeSH Cell Cycle* DNA, Mitochondrial / metabolism* DNA-Binding Proteins / metabolism* Epithelial Cells / physiology* Fluorescent Dyes / analysis HeLa Cells Humans Intravital Microscopy* Microscopy, Fluorescence Mitochondrial Proteins / metabolism* Nucleoproteins / metabolism* Staining and Labeling Transcription Factors / metabolism*
IF 3.998
引用数 10
リソース情報
ヒト・動物細胞 HeLa(RCB0007) HeLa/Fucci2(RCB2867)