RRC ID 34524
著者 Kurata T, Katayama A, Hiramatsu M, Kiguchi Y, Takeuchi M, Watanabe T, Ogasawara H, Ishihama A, Yamamoto K.
タイトル Identification of the set of genes, including nonannotated morA, under the direct control of ModE in Escherichia coli.
ジャーナル J Bacteriol
Abstract ModE is the molybdate-sensing transcription regulator that controls the expression of genes related to molybdate homeostasis in Escherichia coli. ModE is activated by binding molybdate and acts as both an activator and a repressor. By genomic systematic evolution of ligands by exponential enrichment (SELEX) screening and promoter reporter assays, we have identified a total of nine operons, including the hitherto identified modA, moaA, dmsA, and napF operons, of which six were activated by ModE and three were repressed. In addition, two promoters were newly identified and direct transcription of novel genes, referred to as morA and morB, located on antisense strands of yghW and torY, respectively. The morA gene encodes a short peptide, MorA, with an unusual initiation codon. Surprisingly, overexpression of the morA 5' untranslated region exhibited an inhibitory influence on colony formation of E. coli K-12.
巻・号 195(19)
ページ 4496-505
公開日 2013-10-1
DOI 10.1128/JB.00304-13
PII JB.00304-13
PMID 23913318
PMC PMC3807462
MeSH Amino Acid Sequence Base Sequence Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial / physiology* Genome, Bacterial Molecular Sequence Data Promoter Regions, Genetic Protein Binding Regulon Transcription Factors / metabolism*
IF 3.006
引用数 12
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) JW0744-KC