RRC ID 28119
著者 Shinya T, Motoyama N, Ikeda A, Wada M, Kamiya K, Hayafune M, Kaku H, Shibuya N.
タイトル Functional characterization of CEBiP and CERK1 homologs in arabidopsis and rice reveals the presence of different chitin receptor systems in plants.
ジャーナル Plant Cell Physiol
Abstract Chitin is a representative microbe-associated molecular pattern (MAMP) molecule for various fungi and induces immune responses in many plant species. It has been clarified that the chitin signaling in rice requires a receptor kinase OsCERK1 and a receptor-like protein (Os)CEBiP, which specifically binds chitin oligosaccharides. On the other hand, Arabidopsis requires a receptor kinase (At)CERK1 for chitin signaling but it is not clear whether the plant also requires a CEBiP-like molecule for chitin perception/signaling. To clarify the similarity/difference of the chitin receptor in these two model plants, we first characterized CEBiP homologs in Arabidopsis. Only one of three CEBiP homologs, AtCEBiP (LYM2), showed a high-affinity binding for chitin oligosaccharides similar to rice CEBiP. AtCEBiP also represented the major chitin-binding protein in the Arabidopsis membrane. However, the single/triple knockout (KO) mutants of Arabidopsis CEBiP homologs and the overexpressor of AtCEBiP showed chitin-induced defense responses similar to wild-type Arabidopsis, indicating that AtCEBiP is biochemically functional as a chitin-binding protein but does not contribute to signaling. Studies of the chitin binding properties of the ectodomains of At/OsCERK1 and the chimeric receptors consisting of ecto/cytosolic domains of these molecules indicated that AtCERK1 is sufficient for chitin perception by itself.
巻・号 53(10)
ページ 1696-706
公開日 2012-10-1
DOI 10.1093/pcp/pcs113
PII pcs113
PMID 22891159
MeSH Arabidopsis / immunology Arabidopsis / metabolism* Arabidopsis / microbiology Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Chitin / metabolism* Cytosol / metabolism Gene Expression Regulation, Plant Gene Knockout Techniques Oryza / immunology Oryza / metabolism* Oryza / microbiology Plant Proteins / metabolism* Plants, Genetically Modified Protein Serine-Threonine Kinases / metabolism* Protein Structure, Tertiary Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Signal Transduction Tobacco / genetics Tobacco / metabolism
IF 4.062
引用数 101
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 sja16500