RRC ID 37012
著者 Endo H, Yamaguchi M, Tamura T, Nakano Y, Nishikubo N, Yoneda A, Kato K, Kubo M, Kajita S, Katayama Y, Ohtani M, Demura T.
タイトル Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation.
ジャーナル Plant Cell Physiol
Abstract The secondary cell walls of xylem cells, including vessel elements, provide mechanical strength and contribute to the conduction of water and minerals. VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a NAC-domain transcription factor that regulates the expression of genes required for xylem vessel element formation. Transient expression assays using 68 transcription factors that are expressed during xylem vessel differentiation showed that 14 transcription factors, including VND1-VND7, are putative positive regulators of VND7 expression. Electrophoretic mobility shift assays revealed that all seven VND proteins bound to the VND7 promoter region at its SMBE/TERE motif, indicating that VND7 is a direct target of all of the VND transcription factors. Overexpression of VND1-VND5, GATA12 and ANAC075, newly identified transcription factors that function upstream of VND7, resulted in ectopic xylem vessel element formation. These data suggest that VND7 transcription is a regulatory target of multiple classes of transcription factors.
巻・号 56(2)
ページ 242-54
公開日 2015-2-1
DOI 10.1093/pcp/pcu134
PII pcu134
PMID 25265867
MeSH Arabidopsis / cytology Arabidopsis / genetics* Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Base Sequence Cell Differentiation* Enzyme Assays Gene Expression Regulation, Plant* Gene Regulatory Networks Genes, Plant Luciferases / metabolism Models, Biological Molecular Sequence Data Nucleotide Motifs / genetics Plants, Genetically Modified Promoter Regions, Genetic / genetics Protein Binding Transcription Factors / genetics Transcription Factors / metabolism* Up-Regulation Xylem / cytology* Xylem / genetics*
IF 4.062
引用数 64
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda00396 pda00727 pda01468 pda01727 pda01764 pda02680 pda03176 pda03329 pda03693 pda05451 pda05682 pda06010 pda06113 pda06907 pda06930 pda08970 pda09137 pda09868