RRC ID 11403
著者 Taguchi G, Ubukata T, Nozue H, Kobayashi Y, Takahi M, Yamamoto H, Hayashida N.
タイトル Malonylation is a key reaction in the metabolism of xenobiotic phenolic glucosides in Arabidopsis and tobacco.
ジャーナル Plant J
Abstract Tobacco cells (Nicotiana tabacum L.) accumulate harmful naphthols in the form of malonylated glucosides (Taguchi et al., 2005). Here, we showed that the malonylation of glucosides is a system to metabolize xenobiotics and is common to higher plants. Moreover, some plantlets including Arabidopsis thaliana excreted some of the incorporated naphthols into the culture media as their glucosides. In order to analyze the function of malonylation in the metabolism of these xenobiotics, we identified a malonyltransferase gene (At5g39050) responsible for the malonylation of these compounds in A. thaliana. The recombinant enzyme had malonyltransferase activity toward several phenolic glucosides including naphthol glucosides. A knockout mutant of At5g39050 (pmat1) exposed to naphthols accumulated only a few malonylglucosides in the cell, and released larger amounts of simple glucosides into the culture medium. In contrast, forced expression of At5g39050 in the pmat1 mutant resulted in increased malonylglucoside accumulation and decreased glucoside excretion to the media. The results provided clear evidence of whether the release of glucosides or the storage of malonylglucosides was determined by the At5g39050 expression level. A similar event in naphthol metabolism was observed in the tobacco mutant with a suppressed malonyltransferase gene (NtMaT1). These results suggested that malonylation could be a key reaction to separate the way of xenobiotics disposition, that is, release from cell surface or storage in vacuoles.
巻・号 63(6)
ページ 1031-41
公開日 2010-9-1
DOI 10.1111/j.1365-313X.2010.04298.x
PII TPJ4298
PMID 20626660
MeSH Arabidopsis / metabolism* Arabidopsis Proteins / classification Arabidopsis Proteins / genetics Glucosides / metabolism* Molecular Sequence Data Naphthols / metabolism Phenols / metabolism* Phylogeny Plant Proteins / classification Plant Proteins / genetics Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Tobacco / metabolism* Xenobiotics / metabolism*
IF 6.141
引用数 42
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda09072 pda03459 pda11775 pda00075