RRC ID 11401
著者 Nishimura K, Ashida H, Ogawa T, Yokota A.
タイトル A DEAD box protein is required for formation of a hidden break in Arabidopsis chloroplast 23S rRNA.
ジャーナル Plant J
Abstract In plant chloroplasts, the ribosomal RNA (rRNA) of the large subunit of the ribosome undergoes post-maturation fragmentation processing. This processing consists of site-specific cleavage that generates gapped, discontinuous rRNA molecules. However, the molecular mechanism underlying introduction of the gap structure (the 'hidden break') is poorly understood. Here, we found that the DEAD box protein RH39 plays a key role in introduction of the hidden break into the 23S rRNA in Arabidopsis chloroplasts. Genetic screening for an Arabidopsis plant with a drastically reduced level of ribulose-1,5-bisphosphate carboxylase/oxygenase identified an RH39 mutant. The levels of other chloroplast-encoded photosynthetic proteins were also severely reduced. The reductions were not due to a failure of transcription, but rather inefficiency in translation. RNA gel blotting revealed incomplete fragmentation of 23S rRNA in chloroplasts during maturation. In vitro analysis with recombinant RH39 suggested that the protein binds to the adjacent sequence upstream of the hidden break site to exert its function. We propose a molecular mechanism for the RH39-mediated fragmentation processing of 23S rRNA in chloroplasts.
巻・号 63(5)
ページ 766-77
公開日 2010-9-1
DOI 10.1111/j.1365-313X.2010.04276.x
PII TPJ4276
PMID 20561259
MeSH Amino Acid Sequence Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Blotting, Northern Blotting, Western Chloroplasts / metabolism DEAD-box RNA Helicases / genetics* DEAD-box RNA Helicases / metabolism Genes, Essential / genetics Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Molecular Sequence Data Mutation Protein Binding RNA, Chloroplast / genetics RNA, Chloroplast / metabolism RNA, Ribosomal, 23S / genetics* RNA, Ribosomal, 23S / metabolism Recombinant Proteins / metabolism Ribulose-Bisphosphate Carboxylase / genetics Ribulose-Bisphosphate Carboxylase / metabolism Sequence Homology, Amino Acid
IF 6.141
引用数 51
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda13720