RRC ID 21133
著者 Bissoli G, Niñoles R, Fresquet S, Palombieri S, Bueso E, Rubio L, García-Sánchez MJ, Fernández JA, Mulet JM, Serrano R.
タイトル Peptidyl-prolyl cis-trans isomerase ROF2 modulates intracellular pH homeostasis in Arabidopsis.
ジャーナル Plant J
Abstract Intracellular pH must be kept close to neutrality to be compatible with cellular functions, but the mechanisms of pH homeostasis and the responses to intracellular acidification are mostly unknown. In the plant Arabidopsis thaliana, we found that intracellular acid stress generated by weak organic acids at normal external pH induces expression of several chaperone genes, including ROF2, which encodes a peptidyl-prolyl cis-trans isomerase of the FK506-binding protein class. Loss of function of ROF2, and especially double mutation of ROF2 and the closely related gene ROF1, results in acid sensitivity. Over-expression of ROF2 confers tolerance to intracellular acidification by increasing proton extrusion from cells. The activation of the plasma membrane proton pump (H(+) -ATPase) is indirect: over-expression of ROF2 activates K(+) uptake, causing depolarization of the plasma membrane, which activates the electrogenic H(+) pump. The depolarization of ROF2 over-expressing plants explains their tolerance to toxic cations such as lithium, norspermidine and hygromycin B, whose uptake is driven by the membrane potential. As ROF2 induction and intracellular acidification are common consequences of many stresses, this mechanism of pH homeostasis may be of general importance for stress tolerance.
巻・号 70(4)
ページ 704-16
公開日 2012-5-1
DOI 10.1111/j.1365-313X.2012.04921.x
PMID 22268595
MeSH Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Biological Transport Cell Membrane / metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism Homeostasis* Hot Temperature Hydrogen-Ion Concentration Intracellular Space / chemistry Mutation Oligonucleotide Array Sequence Analysis Peptidylprolyl Isomerase / genetics* Peptidylprolyl Isomerase / metabolism Plants, Genetically Modified Potassium / metabolism Proton-Translocating ATPases / metabolism Protons Reverse Transcriptase Polymerase Chain Reaction Rubidium / metabolism Transcriptome
IF 6.141
引用数 38
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda18737