RRC ID 32214
著者 Ito Y, Hirasawa T, Shimizu H.
タイトル Metabolic engineering of Saccharomyces cerevisiae to improve succinic acid production based on metabolic profiling.
ジャーナル Biosci Biotechnol Biochem
Abstract We performed metabolic engineering on the budding yeast Saccharomyces cerevisiae for enhanced production of succinic acid. Aerobic succinic acid production in S. cerevisiae was achieved by disrupting the SDH1 and SDH2 genes, which encode the catalytic subunits of succinic acid dehydrogenase. Increased succinic acid production was achieved by eliminating the ethanol biosynthesis pathways. Metabolic profiling analysis revealed that succinic acid accumulated intracellularly following disruption of the SDH1 and SDH2 genes, which suggests that enhancing the export of intracellular succinic acid outside of cells increases succinic acid production in S. cerevisiae. The mae1 gene encoding the Schizosaccharomyces pombe malic acid transporter was introduced into S. cerevisiae, and as a result, succinic acid production was successfully improved. Metabolic profiling analysis is useful in producing chemicals for metabolic engineering of microorganisms.
巻・号 78(1)
ページ 151-9
公開日 2014-1-1
DOI 10.1080/09168451.2014.877816
PMID 25036498
MeSH Metabolic Engineering / methods* Metabolomics* Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics* Succinic Acid / metabolism*
IF 1.516
引用数 25
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
酵母 BY6313(BY4739) FYG16_m23 BYP803(pNV11)