RRC ID 49285
著者 Kaieda Y, Masuda R, Nishida R, Shimell M, O'Connor MB, Ono H.
タイトル Glue protein production can be triggered by steroid hormone signaling independent of the developmental program in Drosophila melanogaster.
ジャーナル Dev Biol
Abstract Steroid hormones regulate life stage transitions, allowing animals to appropriately follow a developmental timeline. During insect development, the steroid hormone ecdysone is synthesized and released in a regulated manner by the prothoracic gland (PG) and then hydroxylated to the active molting hormone, 20-hydroxyecdysone (20E), in peripheral tissues. We manipulated ecdysteroid titers, through temporally controlled over-expression of the ecdysteroid-inactivating enzyme, CYP18A1, in the PG using the GeneSwitch-GAL4 system in the fruit fly Drosophila melanogaster. We monitored expression of a 20E-inducible glue protein gene, Salivary gland secretion 3 (Sgs3), using a Sgs3:GFP fusion transgene. In wild type larvae, Sgs3-GFP expression is activated at the midpoint of the third larval instar stage in response to the rising endogenous level of 20E. By first knocking down endogenous 20E levels during larval development and then feeding 20E to these larvae at various stages, we found that Sgs3-GFP expression could be triggered at an inappropriate developmental stage after a certain time lag. This stage-precocious activation of Sgs3 required expression of the Broad-complex, similar to normal Sgs3 developmental regulation, and a small level of nutritional input. We suggest that these studies provide evidence for a tissue-autonomic regulatory system for a metamorphic event independent from the primary 20E driven developmental progression.
巻・号 430(1)
ページ 166-176
公開日 2017-10-1
DOI 10.1016/j.ydbio.2017.08.002
PII S0012-1606(17)30246-4
PMID 28782527
PMC PMC5916818
MeSH Animal Structures / drug effects Animal Structures / metabolism Animals Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism* Ecdysterone / metabolism* Glue Proteins, Drosophila / metabolism* Green Fluorescent Proteins / metabolism Larva / drug effects Larva / growth & development Mifepristone / pharmacology Models, Biological Progesterone / analogs & derivatives Signal Transduction Time Factors Transgenes
IF 2.896
引用数 4
リソース情報
ショウジョウバエ DGRC#107870