RRC ID 40070
著者 Condas LA, Ribeiro MG, Muro MD, de Vargas AP, Matsuzawa T, Yazawa K, Siqueira AK, Salerno T, Lara GH, Risseti RM, Ferreira KS, Gonoi T.
タイトル MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL.
ジャーナル Rev Inst Med Trop Sao Paulo
Abstract Nocardia is a ubiquitous microorganism related to pyogranulomatous infection, which is difficult to treat in humans and animals. The occurrence of the disease is on the rise in many countries due to an increase in immunosuppressive diseases and treatments. This report of cases from Brazil presents the genotypic characterization and the antimicrobial susceptibility pattern using the disk-diffusion method and inhibitory minimal concentration with E-test® strips. In summary, this report focuses on infections in young adult men, of which three cases were cutaneous, two pulmonary, one neurological and one systemic. The pulmonary, neurological and systemic cases were attributed to immunosuppressive diseases or treatments. Sequencing analysis of the 16S rRNA segments (1491 bp) identified four isolates of Nocardia farcinica, two isolates of Nocardia nova and one isolate of Nocardia asiatica. N. farcinica was involved in two cutaneous, one systemic and other pulmonary cases; N. nova was involved in one neurological and one pulmonary case; and Nocardia asiatica in one cutaneous case. The disk-diffusion antimicrobial susceptibility test showed that the most effective antimicrobials were amikacin (100%), amoxicillin/clavulanate (100%), cephalexin (100%) and ceftiofur (100%), while isolates had presented most resistance to gentamicin (43%), sulfamethoxazole/trimethoprim (43%) and ampicillin (29%). However, on the inhibitory minimal concentration test (MIC test), only one of the four isolates of Nocardia farcinica was resistant to sulfamethoxazole/trimethoprim.
巻・号 57(3)
ページ 251-6
公開日 2015-1-1
DOI 10.1590/S0036-46652015000300012
PII S0036-46652015000300251
PMID 26200967
PMC PMC4544251
MeSH Adult Animals Anti-Bacterial Agents / pharmacology* Bacterial Typing Techniques Brazil DNA, Bacterial / genetics Disk Diffusion Antimicrobial Tests Humans Male Nocardia / classification Nocardia / genetics* Nocardia / isolation & purification Nocardia Infections / microbiology* RNA, Ribosomal, 16S / genetics
IF 1.46
引用数 2
WOS 分野 TROPICAL MEDICINE
リソース情報
病原微生物 IFM 11096 IFM 11099 IFM 11100 IFM 11128 IFM 11231 IFM 11232 IFM 11285