RRC ID 47437
著者 Sakamoto T, Yoshiki M, Takahashi H, Yoshida M, Ogino Y, Ikeuchi T, Nakamachi T, Konno N, Matsuda K, Sakamoto H.
タイトル Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish.
ジャーナル Sci Rep
Abstract As in osmoregulation, mineralocorticoid signaling is implicated in the control of brain-behavior actions. Nevertheless, the understanding of this role is limited, partly due to the mortality of mineralocorticoid receptor (MR)-knockout (KO) mice due to impaired Na+ reabsorption. In teleost fish, a distinct mineralocorticoid system has only been identified recently. Here, we generated a constitutive MR-KO medaka as the first adult-viable MR-KO animal, since MR expression is modest in osmoregulatory organs but high in the brain of adult medaka as for most teleosts. Hyper- and hypo-osmoregulation were normal in MR-KO medaka. When we studied the behavioral phenotypes based on the central MR localization, however, MR-KO medaka failed to track moving dots despite having an increase in acceleration of swimming. These findings reinforce previous results showing a minor role for mineralocorticoid signaling in fish osmoregulation, and provide the first convincing evidence that MR is required for normal locomotor activity in response to visual motion stimuli, but not for the recognition of these stimuli per se. We suggest that MR potentially integrates brain-behavioral and visual responses, which might be a conserved function of mineralocorticoid signaling through vertebrates. Importantly, this fish model allows for the possible identification of novel aspects of mineralocorticoid signaling.
巻・号 6
ページ 37991
公開日 2016-11-29
DOI 10.1038/srep37991
PII srep37991
PMID 27897263
PMC PMC5126551
MeSH Animals Behavior, Animal Brain / physiology* Eye / metabolism* Locomotion Mineralocorticoids / metabolism* Oryzias / genetics Oryzias / growth & development Oryzias / metabolism* Osmoregulation / physiology* Receptors, Mineralocorticoid / deficiency* Receptors, Mineralocorticoid / genetics Signal Transduction Visual Perception / physiology*
IF 3.998
引用数 16
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
メダカ MR(del2) (MT1050) HdrR-II1 (IB178)