RRC ID 21981
著者 Bunt SM, Monk AC, Siddall NA, Johnston NL, Hime GR.
タイトル GAL4 enhancer traps that can be used to drive gene expression in developing Drosophila spermatocytes.
ジャーナル Genesis
Abstract The Drosophila testis has proven to be a valuable model organ for investigation of germline stem cell (GSC) maintenance and differentiation as well as elucidation of the genetic programs that regulate differentiation of daughter spermatogonia. Development of germ cell specific GAL4 driver transgenes has facilitated investigation of gene function in GSCs and spermatogonia but specific GAL4 tools are not available for analysis of postmitotic spermatogonial differentiation into spermatocytes. We have screened publically available pGT1 strains, a GAL4-encoding gene trap collection, to identify lines that can drive gene expression in late spermatogonia and early spermatocytes. While we were unable to identify any germline-specific drivers, we did identify an insertion in the chiffon locus, which drove expression specifically in early spermatocytes within the germline along with the somatic cyst cells of the testis.
巻・号 50(12)
ページ 914-20
公開日 2012-12-1
DOI 10.1002/dvg.22341
PMID 22926963
MeSH Animals Drosophila / genetics* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Egg Proteins / genetics Enhancer Elements, Genetic* Gene Targeting* Male Spermatocytes / growth & development* Spermatocytes / metabolism Transcription Factors / genetics* Transcription, Genetic* Transgenes*
IF 1.76
引用数 8
WOS 分野 GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ DGRC#111519