| RRC ID |
2393
|
| 著者 |
Inoue K, Ogonuki N, Yamamoto Y, Takano K, Miki H, Mochida K, Ogura A.
|
| タイトル |
Tissue-specific distribution of donor mitochondrial DNA in cloned mice produced by somatic cell nuclear transfer.
|
| ジャーナル |
Genesis
|
| Abstract |
Highly diverse results have been reported for mitochondrial DNA (mtDNA) hetero-plasmy in nuclear-transferred farm animals. In this study, we cloned genetically defined mice and investigated donor mtDNA inheritance following somatic cell cloning. Polymerase chain reaction (PCR) analysis with primers that were specific for either the recipient oocytes or donor cells revealed that the donor mtDNA coexisted with the recipient mtDNA in the brain, liver, kidney, and tail tissues of 96% (24/25) of the adult clones. When the proportion of donor mtDNA in each tissue was measured by allele-specific quantitative PCR and subjected to ANOVA analysis, a tissue-specific mtDNA segregation pattern (P < 0.05) was observed, with the liver containing the highest proportion of donor mtDNA. Therefore, the donor mtDNA was inherited consistently by the cloned offspring, whereas donor mtDNA segregation was not neutral, which is in accordance with previous notions about tissue-specific nuclear control of mtDNA segregation.
|
| 巻・号 |
39(2)
|
| ページ |
79-83
|
| 公開日 |
2004-6-1
|
| DOI |
10.1002/gene.20029
|
| PMID |
15170692
|
| MeSH |
Animals
Brain / metabolism
Cell Nucleus / genetics
Chromosome Segregation
Cloning, Organism*
DNA, Mitochondrial / genetics*
Female
Fibroblasts / physiology
Kidney / metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Inbred DBA
Mitochondria, Liver / metabolism
Nuclear Transfer Techniques*
Oocytes / physiology*
Polymerase Chain Reaction
Species Specificity
Tissue Donors
|
| IF |
1.76
|
| 引用数 |
34
|
|
WOS 分野
|
GENETICS & HEREDITY
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00639 |
