RRC ID 19700
著者 Yu C, Li Y, Li B, Liu X, Hao L, Chen J, Qian W, Li S, Wang G, Bai S, Ye H, Qin H, Shen Q, Chen L, Zhang A, Wang D.
タイトル Molecular analysis of phosphomannomutase (PMM) genes reveals a unique PMM duplication event in diverse Triticeae species and the main PMM isozymes in bread wheat tissues.
ジャーナル BMC Plant Biol
Abstract BACKGROUND:Phosphomannomutase (PMM) is an essential enzyme in eukaryotes. However, little is known about PMM gene and function in crop plants. Here, we report molecular evolutionary and biochemical analysis of PMM genes in bread wheat and related Triticeae species.
RESULTS:Two sets of homologous PMM genes (TaPMM-1 and 2) were found in bread wheat, and two corresponding PMM genes were identified in the diploid progenitors of bread wheat and many other diploid Triticeae species. The duplication event yielding PMM-1 and 2 occurred before the radiation of diploid Triticeae genomes. The PMM gene family in wheat and relatives may evolve largely under purifying selection. Among the six TaPMM genes, the transcript levels of PMM-1 members were comparatively high and their recombinant proteins were all enzymatically active. However, PMM-2 homologs exhibited lower transcript levels, two of which were also inactive. TaPMM-A1, B1 and D1 were probably the main active isozymes in bread wheat tissues. The three isozymes differed from their counterparts in barley and Brachypodium distachyon in being more tolerant to elevated test temperatures.
CONCLUSION:Our work identified the genes encoding PMM isozymes in bread wheat and relatives, uncovered a unique PMM duplication event in diverse Triticeae species, and revealed the main active PMM isozymes in bread wheat tissues. The knowledge obtained here improves the understanding of PMM evolution in eukaryotic organisms, and may facilitate further investigations of PMM function in the temperature adaptability of bread wheat.
巻・号 10
ページ 214
公開日 2010-10-5
DOI 10.1186/1471-2229-10-214
PII 1471-2229-10-214
PMID 20920368
PMC PMC3017832
MeSH Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Evolution, Molecular Gene Dosage Gene Duplication* Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genetic Complementation Test Isoenzymes / genetics Isoenzymes / metabolism Molecular Sequence Data Mutation Phosphotransferases (Phosphomutases) / classification Phosphotransferases (Phosphomutases) / genetics* Phosphotransferases (Phosphomutases) / metabolism Phylogeny Plant Proteins / genetics* Plant Proteins / metabolism Poaceae / classification Poaceae / enzymology Poaceae / genetics Reverse Transcriptase Polymerase Chain Reaction Selection, Genetic Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Species Specificity Triticum / enzymology Triticum / genetics* Yeasts / enzymology Yeasts / genetics
IF 3.497
引用数 11
WOS 分野 PLANT SCIENCES
リソース情報
コムギ KU-2-1 KU-14621-1 KU-14627-1 KU-14610-1 KU-14615-1 KU-14652-1 KU-14656-1 KU-107-1 KU-107-2 KU-12199 KU-21-4 KU-21-6 KU-12966 KU-12790 KU-11488 KU-11-1 KU-11-3 KU-12417