RRC ID 45562
著者 Garcia AM, Ladage ML, Dumesnil DR, Zaman K, Shulaev V, Azad RK, Padilla PA.
タイトル Glucose induces sensitivity to oxygen deprivation and modulates insulin/IGF-1 signaling and lipid biosynthesis in Caenorhabditis elegans.
ジャーナル Genetics
Abstract Diet is a central environmental factor that contributes to the phenotype and physiology of individuals. At the root of many human health issues is the excess of calorie intake relative to calorie expenditure. For example, the increasing amount of dietary sugars in the human diet is contributing to the rise of obesity and type 2 diabetes. Individuals with obesity and type 2 diabetes have compromised oxygen delivery, and thus it is of interest to investigate the impact a high-sugar diet has on oxygen deprivation responses. By utilizing the Caenorhabditis elegans genetic model system, which is anoxia tolerant, we determined that a glucose-supplemented diet negatively impacts responses to anoxia and that the insulin-like signaling pathway, through fatty acid and ceramide synthesis, modulates anoxia survival. Additionally, a glucose-supplemented diet alters lipid localization and initiates a positive chemotaxis response. Use of RNA-sequencing analysis to compare gene expression responses in animals fed either a standard or glucose-supplemented diet revealed that glucose impacts the expression of genes involved with multiple cellular processes including lipid and carbohydrate metabolism, stress responses, cell division, and extracellular functions. Several of the genes we identified show homology to human genes that are differentially regulated in response to obesity or type 2 diabetes, suggesting that there may be conserved gene expression responses between C. elegans fed a glucose-supplemented diet and a diabetic and/or obesity state observed in humans. These findings support the utility of the C. elegans model for understanding the molecular mechanisms regulating dietary-induced metabolic diseases.
巻・号 200(1)
ページ 167-84
公開日 2015-5-1
DOI 10.1534/genetics.115.174631
PII genetics.115.174631
PMID 25762526
PMC PMC4423361
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Carbohydrate Metabolism / genetics Ceramides / biosynthesis Diet Fatty Acids / biosynthesis Gene Expression Profiling Glucose / administration & dosage Glucose / metabolism* Hypoxia / genetics Hypoxia / metabolism* Insulin / metabolism* Insulin-Like Growth Factor I / metabolism* Lipid Metabolism / genetics* Signal Transduction*
IF 4.015
引用数 14
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm420 tm331 tm2031