RRC ID 50863
著者 Aoki-Yoshida A, Saito S, Tsuruta T, Ohsumi A, Tsunoda H, Sonoyama K.
タイトル Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitro.
ジャーナル Biochem Biophys Res Commun
Abstract Orally administered Lactobacillus strains, including L. plantarum No.14 and L. rhamnosus GG, reportedly reduce inflammatory cytokine production in mice. The present study tested our idea that circulating exosomes mediate the action of Lactobacillus strains. The lipopolysaccharide-induced production of TNF-α and IL-6 in vitro was attenuated in peritoneal exudate cells (PECs) isolated from C57BL/6N mice that had been fed L. plantarum No.14. When PECs were cultured for 24 h with exosomes isolated from the serum of mice fed L. plantarum No.14 or L. rhamnosus GG, accumulation of both TNF-α and of the corresponding mRNA was lowered. Growth in the presence of these exosomes also decreased the production of TNF-α and IL-6 by the murine macrophage cell line RAW264.7. In contrast, supplementation with exosome-depleted serum of mice fed L. plantarum No.14 or L. rhamnosus GG failed to affect the production of TNF-α and IL-6 by RAW264.7 cells. When PECs and RAW264.7 cells were cultured for 24 h with PKH67-labeled exosomes isolated from murine serum, fluorescent signal was observed inside the cells, suggesting that these cells incorporate serum exosomes. We propose that the anti-inflammatory activity of orally administered L. plantarum No.14 and L. rhamnosus GG is mediated, at least in part, by circulating exosomes.
巻・号 489(2)
ページ 248-254
公開日 2017-7-22
DOI 10.1016/j.bbrc.2017.05.152
PII S0006-291X(17)31061-6
PMID 28559134
MeSH Administration, Oral Animals Cells, Cultured Cytokines / biosynthesis* Exosomes / immunology Exosomes / microbiology* Female Inflammation / immunology Inflammation / metabolism* Lactobacillus / classification Lactobacillus / immunology* Lipopolysaccharides / administration & dosage Lipopolysaccharides / pharmacology Macrophages / immunology Macrophages / metabolism* Mice Mice, Inbred C57BL RAW 264.7 Cells
IF 2.985
引用数 4
リソース情報
一般微生物 JCM 1149