RRC ID 48762
著者 Tang Y, Zhao CY, Tan ST, Xue HW.
タイトル Arabidopsis Type II Phosphatidylinositol 4-Kinase PI4Kγ5 Regulates Auxin Biosynthesis and Leaf Margin Development through Interacting with Membrane-Bound Transcription Factor ANAC078.
ジャーナル PLoS Genet
Abstract Normal leaf margin development is important for leaf morphogenesis and contributes to diverse leaf shapes in higher plants. We here show the crucial roles of an atypical type II phosphatidylinositol 4-kinase, PI4Kγ5, in Arabidopsis leaf margin development. PI4Kγ5 presents a dynamics expression pattern along with leaf development and a T-DNA mutant lacking PI4Kγ5, pi4kγ5-1, presents serrated leaves, which is resulted from the accelerated cell division and increased auxin concentration at serration tips. Studies revealed that PI4Kγ5 interacts with and phosphorylates a membrane-bound NAC transcription factor, ANAC078. Previous studies demonstrated that membrane-bound transcription factors regulate gene transcription by undergoing proteolytic process to translocate into nucleus, and ANAC078 undergoes proteolysis by cleaving off the transmembrane region and carboxyl terminal. Western blot analysis indeed showed that ANAC078 deleting of carboxyl terminal is significantly reduced in pi4kγ5-1, indicating that PI4Kγ5 is important for the cleavage of ANAC078. This is consistent with the subcellular localization observation showing that fluorescence by GFP-ANAC078 is detected at plasma membrane but not nucleus in pi4kγ5-1 mutant and that expression of ANAC078 deleting of carboxyl terminal, driven by PI4Kγ5 promoter, could rescue the leaf serration defects of pi4kγ5-1. Further analysis showed that ANAC078 suppresses the auxin synthesis by directly binding and regulating the expression of auxin synthesis-related genes. These results indicate that PI4Kγ5 interacts with ANAC078 to negatively regulate auxin synthesis and hence influences cell proliferation and leaf development, providing informative clues for the regulation of in situ auxin synthesis and cell division, as well as the cleavage and functional mechanism of membrane-bound transcription factors.
巻・号 12(8)
ページ e1006252
公開日 2016-8-1
DOI 10.1371/journal.pgen.1006252
PII PGENETICS-D-16-00786
PMID 27529511
PMC PMC4986951
MeSH 1-Phosphatidylinositol 4-Kinase / biosynthesis 1-Phosphatidylinositol 4-Kinase / genetics* Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis Proteins / biosynthesis Arabidopsis Proteins / genetics* Cell Division / genetics Cell Proliferation / genetics DNA, Bacterial / genetics Gene Expression Regulation, Plant Indoleacetic Acids / metabolism* Mutation Plant Development / genetics* Plant Leaves / genetics Plant Leaves / growth & development Plants, Genetically Modified Transcription Factors / biosynthesis Transcription Factors / genetics*
IF 5.175
引用数 9
WOS 分野 GENETICS & HEREDITY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda00454 pda08662