RRC ID |
21566
|
著者 |
Watanabe M, Sato M, Kondo K, Narikawa R, Ikeuchi M.
|
タイトル |
Phycobilisome model with novel skeleton-like structures in a glaucocystophyte Cyanophora paradoxa.
|
ジャーナル |
Biochim Biophys Acta
|
Abstract |
Phycobilisome (PBS) is a photosynthetic antenna supercomplex consisting of a central core subcomplex with several peripheral rods radiating from the core. Subunit structure of PBS was studied in a glaucocystophyte Cyanophora paradoxa strain NIES 547. Subunit composition of PBS was identified by N-terminal sequencing and genes for the subunits were determined by homology search of databases. They included rod linker proteins CpcK1 and CpcK2, rod-core linker proteins CpcG1 and CpcG2, and core linker proteins ApcC1 and ApcC2. Subfractionation by native polyacrylamide gel electrophoresis provided evidence for novel subcomplexes (ApcE/CpcK1/CpcG2/ApcA/ApcB/CpcD and ApcE/CpcK2/CpcG1/ApcA/ApcB), which connect rod and core subcomplexes. These skeleton-like structures may serve as a scaffold of the whole PBS assembly. Different roles of ApcC1 and ApcC2 were also suggested. Based on these findings, structural models for PBS were proposed. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
|
巻・号 |
1817(8)
|
ページ |
1428-35
|
公開日 |
2012-8-1
|
DOI |
10.1016/j.bbabio.2011.11.013
|
PII |
S0005-2728(11)00285-4
|
PMID |
22172737
|
MeSH |
Amino Acid Sequence
Centrifugation, Density Gradient
Cyanophora / chemistry*
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Phycobilisomes / chemistry*
Phylogeny
Protein Subunits
|
IF |
3.411
|
引用数 |
8
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
藻類 |
NIES-547 |