RRC ID 36895
著者 Lin TF, Saiga S, Abe M, Laux T.
タイトル OBE3 and WUS Interaction in Shoot Meristem Stem Cell Regulation.
ジャーナル PLoS One
Abstract The stem cells in the shoot apical meristem (SAM) are the origin of all above ground tissues in plants. In Arabidopsis thaliana, shoot meristem stem cells are maintained by the homeobox transcription factor gene WUS (WUSCHEL) that is expressed in cells of the organizing center underneath the stem cells. In order to identify factors that operate together with WUS in stem cell maintenance, we performed an EMS mutant screen for modifiers of the hypomorphic wus-6 allele. We isolated the oberon3-2 (obe3-2) mutant that enhances stem cell defects in wus-6, but does not affect the putative null allele wus-1. The OBE3 gene encodes a PHD (Plant Homeo Domain) protein that is thought to function in chromatin regulation. Single mutants of OBE3 or its closest homolog OBE4 do not display any defects, whereas the obe3-2 obe4-2 double mutant displays broad growth defects and developmental arrest of seedlings. Transcript levels of WUS and its target gene in the stem cells, CLAVATA3, are reduced in obe3-2. On the other hand, OBE3 and OBE4 transcripts are both indirectly upregulated by ectopic WUS expression. Our results suggest a positive feedback regulation between WUS and OBE3 that contributes to shoot meristem homeostasis.
巻・号 11(5)
ページ e0155657
公開日 2016-1-1
DOI 10.1371/journal.pone.0155657
PII PONE-D-16-06138
PMID 27196372
PMC PMC4873020
MeSH Alleles Arabidopsis / genetics* Arabidopsis / growth & development Arabidopsis Proteins / genetics* Carrier Proteins / genetics* Chromatin / metabolism Chromosome Mapping Feedback, Physiological Flowers / genetics Gene Expression Regulation, Plant* Genotype Homeodomain Proteins / genetics* Meristem / cytology Meristem / growth & development* Mutagenesis Mutation Phenotype Polymerase Chain Reaction Protein Domains RNA, Messenger / metabolism Seedlings / genetics Seedlings / growth & development Stem Cells / cytology* Transcription Factors
IF 2.74
引用数 2
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda2153