RRC ID 30562
著者 Kojima Y, Wakita J, Inayoshi Y, Suzuki R, Yamada Y, Kaneoka H, Nishijima K, Iijima S.
タイトル Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase.
ジャーナル J Biosci Bioeng
Abstract Human erythropoietin produced in the egg white of chimeric chicken contains N-glycan with lower amounts of terminal galactose and sialic acid; therefore, the chicken galactosyltransferase gene was introduced together with the human erythropoietin gene by a retroviral vector. We found that erythropoietin accumulated in the egg white was partially galactosylated.
巻・号 117(6)
ページ 676-9
公開日 2014-6-1
DOI 10.1016/j.jbiosc.2013.11.010
PII S1389-1723(13)00432-5
PMID 24355574
MeSH Animals Animals, Genetically Modified / genetics Avian Proteins / biosynthesis Avian Proteins / genetics Chick Embryo Chickens / genetics* Egg Proteins / metabolism Erythropoietin / genetics Erythropoietin / metabolism* Galactose / metabolism* Galactosyltransferases / biosynthesis Galactosyltransferases / genetics Glycosylation Humans Plasmids Protein Processing, Post-Translational* Vesiculovirus / genetics
IF 2.366
引用数 5
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ニワトリ・ウズラ