RRC ID 47672
著者 Nakazawa M, Matsubara H, Matsushita Y, Watanabe M, Vo N, Yoshida H, Yamaguchi M, Kataoka T.
タイトル The Human Bcl-2 Family Member Bcl-rambo Localizes to Mitochondria and Induces Apoptosis and Morphological Aberrations in Drosophila.
ジャーナル PLoS One
Abstract Bcl-2 family proteins play a central role in regulating apoptosis. We previously reported that human Bcl-rambo, also termed BCL2L13, localized to mitochondria and induced apoptosis when overexpressed in human embryonic kidney 293T cells. However, the physiological function of Bcl-rambo currently remains unclear. In the present study, human Bcl-rambo was ectopically expressed in Drosophila melanogaster. Bcl-rambo mainly localized to the mitochondria of Drosophila Schneider 2 (S2) cells. The overexpression of Bcl-rambo, but not Bcl-rambo lacking a C-terminal transmembrane domain, induced apoptosis in S2 cells. Moreover, the ectopic expression of Bcl-rambo by a GAL4-UAS system induced aberrant morphological changes characterized by atrophied wing, split thorax, and rough eye phenotypes. Bcl-rambo induced the activation of effector caspases in eye imaginal discs. The rough eye phenotype induced by Bcl-rambo was partly rescued by the co-expression of p35, Diap1, and Diap2. By using this Drosophila model, we showed that human Bcl-rambo interacted genetically with Drosophila homologues of adenine nucleotide translocators and the autophagy-related 8 protein. The results of the present study demonstrated that human Bcl-rambo localized to mitochondria and at least regulated an apoptosis signaling pathway in Drosophila.
巻・号 11(6)
ページ e0157823
公開日 2016-6-27
DOI 10.1371/journal.pone.0157823
PII PONE-D-15-24486
PMID 27348811
PMC PMC4922555
MeSH Animals Apoptosis* Autophagy-Related Protein 8 Family / genetics Cell Line Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Epistasis, Genetic Humans Inhibitor of Apoptosis Proteins / genetics Inhibitor of Apoptosis Proteins / metabolism Mitochondria / metabolism* Mitochondrial ADP, ATP Translocases / genetics Phenotype* Protein Transport Proto-Oncogene Proteins c-bcl-2 / genetics* Proto-Oncogene Proteins c-bcl-2 / metabolism
IF 2.74
引用数 15
WOS 分野 CELL BIOLOGY
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