RRC ID 28116
著者 Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y, Huang S.
タイトル Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments.
ジャーナル Plant J
Abstract The organization of the actin cytoskeleton has been implicated in sclerenchyma development. However, the molecular mechanisms linking the actin cytoskeleton to this process remain poorly understood. In particular, there have been no studies showing that direct genetic manipulation of the actin cytoskeleton affects sclerenchyma development. Villins belong to the villin/gelsolin/fragmin superfamily and are versatile actin-modifying proteins. Several recent studies have implicated villins in tip growth of single cells, but how villins act in multicellular plant development remains largely unknown. Here, we found that two closely related villin isovariants from Arabidopsis, VLN2 and VLN3, act redundantly in sclerenchyma development. Detailed analysis of cross-sections from inflorescence stems of vln2 vln3 double mutant plants revealed a reduction in stem size and in the number of vascular bundles; however, no defects in synthesis of the secondary cell wall were detected. Surprisingly, the vln2 vln3 double mutation did not affect cell elongation of inter-fascicular fibers. Biochemical analyses showed that recombinant VLN2 was able to cap, sever and bundle actin filaments, similar to VLN3. Consistent with these biochemical activities, loss of function of VLN2 and VLN3 resulted in a decrease in the amount of F-actin and actin bundles in plant cells. Collectively, our findings demonstrate that VLN2 and VLN3 act redundantly in sclerenchyma development via bundling of actin filaments.
巻・号 71(6)
ページ 962-75
公開日 2012-9-1
DOI 10.1111/j.1365-313X.2012.05044.x
PMID 22563899
MeSH Actin Cytoskeleton / metabolism* Actins / metabolism Arabidopsis / cytology Arabidopsis / genetics* Arabidopsis / growth & development* Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Wall / metabolism Inflorescence / cytology Inflorescence / genetics Inflorescence / growth & development Inflorescence / metabolism Microfilament Proteins / genetics Microfilament Proteins / metabolism Mutagenesis, Insertional Phenotype Plant Stems / cytology Plant Stems / genetics Plant Stems / growth & development Plant Stems / metabolism Plant Vascular Bundle / cytology Plant Vascular Bundle / genetics Plant Vascular Bundle / growth & development Plant Vascular Bundle / metabolism Recombinant Proteins
IF 6.141
引用数 32
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda07649