RRC ID 29424
著者 Morita S, Tagai C, Shiraishi T, Miyaji K, Iwamuro S.
タイトル Differential mode of antimicrobial actions of arginine-rich and lysine-rich histones against Gram-positive Staphylococcus aureus.
ジャーナル Peptides
Abstract We previously reported the activities and modes of action of arginine (Arg)-rich histones H3 and H4 against Gram-negative bacteria. In the present study, we investigated the properties of the Arg-rich histones against Gram-positive bacteria in comparison with those of lysine (Lys)-rich histone H2B. In a standard microdilution assay, calf thymus histones H2B, H3, and H4 showed growth inhibitory activity against Staphylococcus aureus with minimum effective concentration values of 4.0, 4.0, and 5.6 μM, respectively. Laser confocal microscopic analyses revealed that both the Arg-rich and Lys-rich histones associated with the surface of S. aureus. However, while the morphology of S. aureus treated with histone H2B appeared intact, those treated with the histones H3 and H4 closely resembled each other, and the cells were blurred. Electrophoretic mobility shift assay results revealed these histones have binding affinity to lipoteichoic acid (LTA), one of major cell surface components of Gram-positive bacteria. Scanning electron microscopic analyses demonstrated that while histone H2B elicited no obvious changes in cell morphology, histones H3 and H4 disrupted the cell membrane structure with bleb formation in a manner similar to general antimicrobial peptides. Consequently, our results suggest that bacterial cell surface LTA initially attracts both the Arg- and Lys-rich histones, but the modes of antimicrobial action of these histones are different; the former involves cell membrane disruption and the latter involves the cell integrity disruption.
巻・号 48
ページ 75-82
公開日 2013-10-1
DOI 10.1016/j.peptides.2013.07.025
PII S0196-9781(13)00265-9
PMID 23932939
MeSH Animals Anti-Infective Agents / chemistry Anti-Infective Agents / metabolism Anti-Infective Agents / pharmacology* Arginine / chemistry Cattle Cell Membrane / metabolism Cell Membrane / ultrastructure Histones / chemistry Histones / metabolism Histones / pharmacology* Humans Kinetics Lipopolysaccharides / metabolism Lysine / chemistry Microscopy, Electrochemical, Scanning Protein Binding Staphylococcal Infections / drug therapy Staphylococcal Infections / pathology Staphylococcus aureus / drug effects Staphylococcus aureus / ultrastructure Teichoic Acids / metabolism
IF 2.843
引用数 25
WOS 分野 PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 2874