RRC ID |
49299
|
著者 |
Saito Y, Sugimura T, Yanagawa Y.
|
タイトル |
Comparisons of Neuronal and Excitatory Network Properties between the Rat Brainstem Nuclei that Participate in Vertical and Horizontal Gaze Holding.
|
ジャーナル |
eNeuro
|
Abstract |
Gaze holding is primarily controlled by neural structures including the prepositus hypoglossi nucleus (PHN) for horizontal gaze and the interstitial nucleus of Cajal (INC) for vertical and torsional gaze. In contrast to the accumulating findings of the PHN, there is no report regarding the membrane properties of INC neurons or the local networks in the INC. In this study, to verify whether the neural structure of the INC is similar to that of the PHN, we investigated the neuronal and network properties of the INC using whole-cell recordings in rat brainstem slices. Three types of afterhyperpolarization (AHP) profiles and five firing patterns observed in PHN neurons were also observed in INC neurons. However, the overall distributions based on the AHP profile and the firing patterns of INC neurons were different from those of PHN neurons. The application of burst stimulation to a nearby site of a recorded INC neuron induced an increase in the frequency of spontaneous EPSCs. The duration of the increased EPSC frequency of INC neurons was not significantly different from that of PHN neurons. The percent of duration reduction induced by a Ca2+-permeable AMPA (CP-AMPA) receptor antagonist was significantly smaller in the INC than in the PHN. These findings suggest that local excitatory networks that activate sustained EPSC responses also exist in the INC, but their activation mechanisms including the contribution of CP-AMPA receptors differ between the INC and the PHN.
|
巻・号 |
4(5)
|
公開日 |
2017-9-13
|
DOI |
10.1523/ENEURO.0180-17.2017
|
PII |
eN-NWR-0180-17
|
PMID |
28966973
|
PMC |
PMC5616193
|
MeSH |
Animals
Animals, Newborn
Brain Stem / cytology*
Brain Stem / metabolism*
Cell Nucleus / drug effects
Cell Nucleus / genetics
Choline O-Acetyltransferase / genetics
Choline O-Acetyltransferase / metabolism
Electric Stimulation
Excitatory Postsynaptic Potentials / genetics
Fixation, Ocular / physiology*
Glycine Agents / pharmacology
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Nerve Net / drug effects
Nerve Net / physiology*
Neural Inhibition / drug effects
Neural Inhibition / genetics
Neurons / drug effects
Neurons / physiology*
Patch-Clamp Techniques
Rats
Rats, Transgenic
Rats, Wistar
Spermine / analogs & derivatives
Spermine / pharmacology
Strychnine / pharmacology
Vesicular Inhibitory Amino Acid Transport Proteins / genetics
Vesicular Inhibitory Amino Acid Transport Proteins / metabolism
|
IF |
3.544
|
引用数 |
2
|
リソース情報 |
ラット |
W-Tg(Slc32a1-YFP*)2Yyan (strainID=908) |