RRC ID 45602
著者 Katsumata K, Hirayasu A, Miyoshi J, Nishi E, Ichikawa K, Tateho K, Wakuda A, Matsuhara H, Yamamoto A.
タイトル A Taz1- and Microtubule-Dependent Regulatory Relationship between Telomere and Centromere Positions in Bouquet Formation Secures Proper Meiotic Divisions.
ジャーナル PLoS Genet
Abstract During meiotic prophase, telomeres cluster, forming the bouquet chromosome arrangement, and facilitate homologous chromosome pairing. In fission yeast, bouquet formation requires switching of telomere and centromere positions. Centromeres are located at the spindle pole body (SPB) during mitotic interphase, and upon entering meiosis, telomeres cluster at the SPB, followed by centromere detachment from the SPB. Telomere clustering depends on the formation of the microtubule-organizing center at telomeres by the linker of nucleoskeleton and cytoskeleton complex (LINC), while centromere detachment depends on disassembly of kinetochores, which induces meiotic centromere formation. However, how the switching of telomere and centromere positions occurs during bouquet formation is not fully understood. Here, we show that, when impaired telomere interaction with the LINC or microtubule disruption inhibited telomere clustering, kinetochore disassembly-dependent centromere detachment and accompanying meiotic centromere formation were also inhibited. Efficient centromere detachment required telomere clustering-dependent SPB recruitment of a conserved telomere component, Taz1, and microtubules. Furthermore, when artificial SPB recruitment of Taz1 induced centromere detachment in telomere clustering-defective cells, spindle formation was impaired. Thus, detachment of centromeres from the SPB without telomere clustering causes spindle impairment. These findings establish novel regulatory mechanisms, which prevent concurrent detachment of telomeres and centromeres from the SPB during bouquet formation and secure proper meiotic divisions.
巻・号 12(9)
ページ e1006304
公開日 2016-9-1
DOI 10.1371/journal.pgen.1006304
PII PGENETICS-D-16-00825
PMID 27611693
PMC PMC5017736
MeSH Centromere / genetics* Prophase* Protein Binding Schizosaccharomyces / genetics Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spindle Pole Bodies / genetics Spindle Pole Bodies / metabolism* Telomere / genetics* Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism*
IF 5.175
引用数 2
WOS 分野 GENETICS & HEREDITY
リソース情報
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