RRC ID 38395
著者 Yoshizaki H, Kuwajima Y, Minato H, Kiyokawa E.
タイトル Regulation of Ripply1 expression in MDCK organoids.
ジャーナル Biochem Biophys Res Commun
Abstract Epithelial organs are made of a well-polarized monolayer of epithelial cells, and their morphology must be maintained for their proper function. To examine the genes that are specifically expressed in the late stages of cystogenesis and are involved in maintaining the morphology of the mature cysts, we performed a microarray analysis comparing the mRNA expression between the early and late stages of Madin-Darby Canine Kidney (MDCK) cystogenesis. We found that one of the gene candidates, Ripply1, was expressed higher in the late stages, and its expression was also transiently much higher in the early stages. Although the protein expression showed similar kinetics, depletion of Ripply1 had only a slight effect on organoid growth. Unexpectedly, we found that the Ripply1 protein is degraded by the proteasome system. Mutant analysis suggests that Ripply1 is not ubiquitinated directly, but rather is degraded only after binding to Transducin-like Enhancer of Split (TLE)1, a transcriptional repressor. Ripply1 is degraded in the nucleus, and this degradation is inhibited during the mitosis. These data indicate for the first time that Ripply1 expression is regulated at the protein level.
巻・号 468(1-2)
ページ 337-42
公開日 2015-12-4
DOI 10.1016/j.bbrc.2015.10.099
PII S0006-291X(15)30799-3
PMID 26514726
MeSH Animals Dogs Gene Expression Regulation Madin Darby Canine Kidney Cells / cytology* Madin Darby Canine Kidney Cells / metabolism Mitosis Nuclear Proteins / genetics* Nuclear Proteins / metabolism Organoids / cytology* Organoids / metabolism Proteolysis RNA Interference RNA, Small Interfering / genetics Repressor Proteins / genetics* Repressor Proteins / metabolism
IF 2.985
引用数 3
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS
リソース情報
ヒト・動物細胞 MDCK(RCB0995)