RRC ID 48854
著者 Wang CH, Huang YC, Chen PY, Cheng YJ, Kao HH, Pi H, Chien CT.
タイトル USP5/Leon deubiquitinase confines postsynaptic growth by maintaining ubiquitin homeostasis through Ubiquilin.
ジャーナル Elife
Abstract Synapse formation and growth are tightly controlled processes. How synaptic growth is terminated after reaching proper size remains unclear. Here, we show that Leon, the Drosophila USP5 deubiquitinase, controls postsynaptic growth. In leon mutants, postsynaptic specializations of neuromuscular junctions are dramatically expanded, including the subsynaptic reticulum, the postsynaptic density, and the glutamate receptor cluster. Expansion of these postsynaptic features is caused by a disruption of ubiquitin homeostasis with accumulation of free ubiquitin chains and ubiquitinated substrates in the leon mutant. Accumulation of Ubiquilin (Ubqn), the ubiquitin receptor whose human homolog ubiquilin 2 is associated with familial amyotrophic lateral sclerosis, also contributes to defects in postsynaptic growth and ubiquitin homeostasis. Importantly, accumulations of postsynaptic proteins cause different aspects of postsynaptic overgrowth in leon mutants. Thus, the deubiquitinase Leon maintains ubiquitin homeostasis and proper Ubqn levels, preventing postsynaptic proteins from accumulation to confine postsynaptic growth.
巻・号 6
公開日 2017-5-10
DOI 10.7554/eLife.26886
PII e26886
PMID 28489002
PMC PMC5438252
MeSH Animals Carrier Proteins / metabolism* Cell Cycle Proteins / metabolism* Drosophila Drosophila Proteins / metabolism* Homeostasis Post-Synaptic Density / metabolism* Receptors, Glutamate / metabolism* Ubiquitin / metabolism* Ubiquitin-Specific Proteases / metabolism*
IF 7.08
引用数 10
リソース情報
ショウジョウバエ 5848R-3