RRC ID 50305
著者 Evrard M, Kwok IWH, Chong SZ, Teng KWW, Becht E, Chen J, Sieow JL, Penny HL, Ching GC, Devi S, Adrover JM, Li JLY, Liong KH, Tan L, Poon Z, Foo S, Chua JW, Su IH, Balabanian K, Bachelerie F, Biswas SK, Larbi A, Hwang WYK, Madan V, Koeffler HP, Wong SC, Newell EW, Hidalgo A, Ginhoux F, Ng LG.
タイトル Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions.
ジャーナル Immunity
Abstract Neutrophils are specialized innate cells that require constant replenishment from proliferative bone marrow (BM) precursors as a result of their short half-life. Although it is established that neutrophils are derived from the granulocyte-macrophage progenitor (GMP), the differentiation pathways from GMP to functional mature neutrophils are poorly defined. Using mass cytometry (CyTOF) and cell-cycle-based analysis, we identified three neutrophil subsets within the BM: a committed proliferative neutrophil precursor (preNeu) which differentiates into non-proliferating immature neutrophils and mature neutrophils. Transcriptomic profiling and functional analysis revealed that preNeu require the C/EBPε transcription factor for their generation from the GMP, and their proliferative program is substituted by a gain of migratory and effector function as they mature. preNeus expand under microbial and tumoral stress, and immature neutrophils are recruited to the periphery of tumor-bearing mice. In summary, our study identifies specialized BM granulocytic populations that ensure supply under homeostasis and stress responses.
巻・号 48(2)
ページ 364-379.e8
公開日 2018-2-20
DOI 10.1016/j.immuni.2018.02.002
PII S1074-7613(18)30038-4
PMID 29466759
MeSH Animals Bone Marrow Cells / immunology Bone Marrow Cells / physiology* CCAAT-Enhancer-Binding Proteins / physiology Cell Lineage Cell Movement Cell Proliferation Cells, Cultured Gene Expression Profiling Humans Mice Neoplasms, Experimental / immunology Neutrophils / immunology Neutrophils / physiology*
IF 22.553
引用数 72
リソース情報
実験動物マウス RBRC02704