RRC ID 48157
著者 Kim SJ, Lee JC, Han SI, Whang KS.
タイトル Halobacillus salicampi sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment.
ジャーナル Antonie Van Leeuwenhoek
Abstract A Gram-positive, moderately halophilic bacterium, designated strain TGS-15(T), was isolated from the sediment of a solar saltern pond located in Shinan, Korea. Strain TGS-15(T) was found to be a strictly aerobic, non-motile rod which can grow at pH 6.0-10.0 (optimum, pH 9.0), at 20-35 °C (optimum, 28 °C) and at salinities of 1-20 % (w/v) NaCl (optimum, 9 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TGS-15(T) belongs to the genus Halobacillus, with sequence similarity of 98.5-96.0 % to known type strains, showing high sequence similarity to Halobacillus locisalis MSS-155(T) (98.5 %), Halobacillus faecis IGA7-4(T) (98.2 %) and Halobacillus alkaliphilus FP5(T) (98.0 %), and less than 98.0 % sequence similarity to other currently recognised type strains of the genus. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and an unidentified lipid. The cell wall peptidoglycan was found to be based on L-Orn-D-Asp, the predominant isoprenoid quinone was identified as menaquinone-7 (MK-7) and the major fatty acids were identified as anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:1 ω7c alcohol. The DNA G+C content of this novel isolate was determined to be 45.3 mol %. Levels of DNA:DNA relatedness between strain TGS-15(T) and the type strains of 13 other species of the genus ranged from 52 to 9 %. On the basis of the polyphasic analysis conducted in this study, strain TGS-15(T) is concluded to represent a novel species of the genus Halobacillus, for which the name Halobacillus salicampi sp. nov. is proposed. The type strain is TGS-15(T) (=KACC 18264(T) = NBRC 110640(T)).
巻・号 109(5)
ページ 713-20
公開日 2016-5-1
DOI 10.1007/s10482-016-0672-y
PII 10.1007/s10482-016-0672-y
PMID 26942918
MeSH Bacterial Typing Techniques DNA, Bacterial / genetics DNA, Ribosomal / genetics Geologic Sediments / chemistry Geologic Sediments / microbiology* Halobacillus / classification* Halobacillus / genetics Halobacillus / isolation & purification* Halobacillus / physiology Phenotype Phylogeny Ponds / microbiology* Republic of Korea Salinity Water Microbiology
IF 2.674
引用数 4
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 12772 JCM 14154