RRC ID 49224
著者 Yao L, Wang S, Westholm JO, Dai Q, Matsuda R, Hosono C, Bray S, Lai EC, Samakovlis C.
タイトル Genome-wide identification of Grainy head targets in Drosophila reveals regulatory interactions with the POU domain transcription factor Vvl.
ジャーナル Development
Abstract Grainy head (Grh) is a conserved transcription factor (TF) controlling epithelial differentiation and regeneration. To elucidate Grh functions we identified embryonic Grh targets by ChIP-seq and gene expression analysis. We show that Grh controls hundreds of target genes. Repression or activation correlates with the distance of Grh-binding sites to the transcription start sites of its targets. Analysis of 54 Grh-responsive enhancers during development and upon wounding suggests cooperation with distinct TFs in different contexts. In the airways, Grh-repressed genes encode key TFs involved in branching and cell differentiation. Reduction of the POU domain TF Ventral veins lacking (Vvl) largely ameliorates the airway morphogenesis defects of grh mutants. Vvl and Grh proteins additionally interact with each other and regulate a set of common enhancers during epithelial morphogenesis. We conclude that Grh and Vvl participate in a regulatory network controlling epithelial maturation.
巻・号 144(17)
ページ 3145-3155
公開日 2017-9-1
DOI 10.1242/dev.143297
PII dev.143297
PMID 28760809
PMC PMC5627367
MeSH Animals Base Sequence Binding Sites / genetics DNA-Binding Proteins / metabolism* Drosophila Proteins / chemistry* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism* Embryo, Nonmammalian / metabolism Epithelium / metabolism Gene Expression Regulation, Developmental Genes, Reporter Genome, Insect* Immunity, Innate / genetics Morphogenesis / genetics Organ Specificity / genetics POU Domain Factors / chemistry* POU Domain Factors / metabolism Protein Binding Protein Domains Respiratory System / metabolism Response Elements / genetics Transcription Factors / metabolism*
IF 5.611
引用数 7
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