RRC ID 51415
著者 Cottee PA, Cole T, Schultz J, Hoang HD, Vibbert J, Han SM, Miller MA.
タイトル The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development.
ジャーナル Development
Abstract VAMP/synaptobrevin-associated proteins (VAPs) contain an N-terminal major sperm protein domain (MSPd) that is associated with amyotrophic lateral sclerosis. VAPs have an intracellular housekeeping function, as well as an extracellular signaling function mediated by the secreted MSPd. Here we show that the C. elegans VAP homolog VPR-1 is essential for gonad development. vpr-1 null mutants are maternal effect sterile due to arrested gonadogenesis following embryo hatching. Somatic gonadal precursor cells and germ cells fail to proliferate fully and complete their respective differentiation programs. Maternal or zygotic vpr-1 expression is sufficient to induce gonadogenesis and fertility. Genetic mosaic and cell type-specific expression studies indicate that vpr-1 activity is important in the nervous system, germ line and intestine. VPR-1 acts in parallel to Notch signaling, a key regulator of germline stem cell proliferation and differentiation. Neuronal vpr-1 expression is sufficient for gonadogenesis induction during a limited time period shortly after hatching. These results support the model that the secreted VPR-1 MSPd acts at least in part on gonadal sheath cell precursors in L1 to early L2 stage hermaphrodites to permit gonadogenesis.
巻・号 144(12)
ページ 2187-2199
公開日 2017-6-15
DOI 10.1242/dev.152207
PII 144/12/2187
PMID 28634273
PMC PMC5482997
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Differentiation Female Gene Knockout Techniques Genome, Helminth Germ Cells / cytology Germ Cells / metabolism Gonads / growth & development* Gonads / metabolism* Intestinal Mucosa / metabolism Intestines / growth & development Male Membrane Proteins / deficiency Membrane Proteins / genetics Membrane Proteins / metabolism* Models, Biological Mosaicism Neurogenesis Organogenesis Receptors, Notch / genetics Receptors, Notch / metabolism Signal Transduction
IF 5.611
引用数 3
リソース情報
線虫 tm1411