RRC ID |
35770
|
著者 |
Saka K, Tadenuma M, Nakade S, Tanaka N, Sugawara H, Nishikawa K, Ichiyoshi N, Kitagawa M, Mori H, Ogasawara N, Nishimura A.
|
タイトル |
A complete set of Escherichia coli open reading frames in mobile plasmids facilitating genetic studies.
|
ジャーナル |
DNA Res
|
Abstract |
To facilitate genetic studies of Escherichia coli, we constructed a complete set of mobile plasmid clones of intact open reading frames (ORFs). Their expression is strictly controlled by Ptac / lacI(q). The plasmids carrying each ORF were introduced into an F+ recA strain and stored in 96-well microtiter plates. In this way, 96 clones can be transferred simultaneously to F- bacteria using the conjugative system. This provides a convenient procedure for systematic identification of ORFs that suppress or complement mutations. We created two types of clone sets: the original set contained individual clones in 45 microtiter plates, and a second set contained pools of 48 clones stored in a single microtiter plate. Using these clone sets, we have identified 403 genes that can correct in trans the temperature-sensitive defect of cell division mutants, which would suggest multiple global regulators for bacterial cell division.
|
巻・号 |
12(1)
|
ページ |
63-8
|
公開日 |
2005-2-28
|
DOI |
10.1093/dnares/12.1.63
|
PMID |
16106753
|
MeSH |
Base Sequence
Cell Division / genetics
Cloning, Molecular
Escherichia coli / genetics*
Genes, Bacterial
Genetic Complementation Test / methods
Models, Genetic
Molecular Sequence Data
Open Reading Frames / genetics*
Plasmids*
|
IF |
4.009
|
引用数 |
62
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
原核生物(大腸菌) |
Mobile plasmid Collection |