RRC ID 49310
著者 Abdelkareem A, Thagun C, Nakayasu M, Mizutani M, Hashimoto T, Shoji T.
タイトル Jasmonate-induced biosynthesis of steroidal glycoalkaloids depends on COI1 proteins in tomato.
ジャーナル Biochem Biophys Res Commun
Abstract In tomato, perception of jasmonates by a receptor complex, which includes the F-box protein CORONATINE INSENSITIVE 1 (COI1), elicits biosynthesis of defensive steroidal glycoalkaloids (SGAs) via a jasmonate-responsive ERF transcription factor, JRE4/GAME9. Although JRE4 is upregulated by jasmonate and induces the expression of many metabolic genes involved in SGA biosynthesis, it is not known whether JRE4 alone is sufficient for increased SGA biosynthesis upon activation of jasmonate signaling. Here, we show that application of methyl jasmonate induces the expression of JRE4 and SGA biosynthesis genes in leaves and hairy roots of wild-type tomato, but not in jasmonic acid insensitive 1 (jai1), a loss-of-function mutant allele of the tomato COI1 gene. Induced overexpression of JRE4 increased the expression of SGA biosynthesis genes in transgenic hairy roots of both wild-type tomato and the jai1 mutant, suggesting that JRE4 is the primary transcription factor that functions downstream of the jasmonate signaling pathway.
巻・号 489(2)
ページ 206-210
公開日 2017-7-22
DOI 10.1016/j.bbrc.2017.05.132
PII S0006-291X(17)31025-2
PMID 28554842
MeSH Alkaloids / biosynthesis* Alkaloids / chemistry Cyclopentanes / administration & dosage Cyclopentanes / pharmacology* Oxylipins / administration & dosage Oxylipins / pharmacology* Phytosterols / biosynthesis* Phytosterols / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Solanum lycopersicum / drug effects* Solanum lycopersicum / metabolism*
IF 2.985
引用数 9
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS
リソース情報
トマト TOMJPF00001