RRC ID 45595
著者 Liu O, Grant BD.
タイトル Basolateral Endocytic Recycling Requires RAB-10 and AMPH-1 Mediated Recruitment of RAB-5 GAP TBC-2 to Endosomes.
ジャーナル PLoS Genet
Abstract The small GTPase RAB-5/Rab5 is a master regulator of the early endosome, required for a myriad of coordinated activities, including the degradation and recycling of internalized cargo. Here we focused on the recycling function of the early endosome and the regulation of RAB-5 by GAP protein TBC-2 in the basolateral C. elegans intestine. We demonstrate that downstream basolateral recycling regulators, GTPase RAB-10/Rab10 and BAR domain protein AMPH-1/Amphiphysin, bind to TBC-2 and help to recruit it to endosomes. In the absence of RAB-10 or AMPH-1 binding to TBC-2, RAB-5 membrane association is abnormally high and recycling cargo is trapped in early endosomes. Furthermore, the loss of TBC-2 or AMPH-1 leads to abnormally high spatial overlap of RAB-5 and RAB-10. Taken together our results indicate that RAB-10 and AMPH-1 mediated down-regulation of RAB-5 is an important step in recycling, required for cargo exit from early endosomes and regulation of early endosome-recycling endosome interactions.
巻・号 11(9)
ページ e1005514
公開日 2015-1-1
DOI 10.1371/journal.pgen.1005514
PII PGENETICS-D-15-00956
PMID 26393361
PMC PMC4578947
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Endocytosis / genetics Endosomes / genetics Endosomes / metabolism GTPase-Activating Proteins / genetics* GTPase-Activating Proteins / metabolism Gene Expression Regulation / genetics Intestinal Mucosa / metabolism Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Protein Transport / genetics RNA Interference Vesicular Transport Proteins / genetics* Vesicular Transport Proteins / metabolism rab GTP-Binding Proteins / genetics* rab GTP-Binding Proteins / metabolism
IF 5.175
引用数 10
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm2241 tm1060