RRC ID 33116
著者 Matsumoto Y, Ishii M, Ishii K, Miyaguchi W, Horie R, Inagaki Y, Hamamoto H, Tatematsu K, Uchino K, Tamura T, Sezutsu H, Sekimizu K.
タイトル Transgenic silkworms expressing human insulin receptors for evaluation of therapeutically active insulin receptor agonists.
ジャーナル Biochem Biophys Res Commun
Abstract We established a transgenic silkworm strain expressing the human insulin receptor (hIR) using the GAL4/UAS system. Administration of human insulin to transgenic silkworms expressing hIR decreased hemolymph sugar levels and facilitated Akt phosphorylation in the fat body. The decrease in hemolymph sugar levels induced by injection of human insulin in the transgenic silkworms expressing hIR was blocked by co-injection of wortmannin, a phosphoinositide 3-kinase inhibitor. Administration of bovine insulin, an hIR ligand, also effectively decreased sugar levels in the transgenic silkworms. These findings indicate that functional hIRs that respond to human insulin were successfully induced in the transgenic silkworms. We propose that the humanized silkworm expressing hIR is useful for in vivo evaluation of the therapeutic activities of insulin receptor agonists.
巻・号 455(3-4)
ページ 159-64
公開日 2014-12-12
DOI 10.1016/j.bbrc.2014.10.143
PII S0006-291X(14)01972-X
PMID 25449269
MeSH Amino Acid Sequence Androstadienes / chemistry Animals Animals, Genetically Modified Antigens, CD / biosynthesis* Bombyx / genetics* Cattle Disease Models, Animal Drug Discovery Glucose / analysis Hemolymph / drug effects* Humans Insulin / chemistry* Ligands Molecular Sequence Data Phosphoinositide-3 Kinase Inhibitors Phosphorylation Receptor, Insulin / agonists* Receptor, Insulin / biosynthesis* Signal Transduction Wortmannin
IF 2.985
引用数 13
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
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