RRC ID |
51396
|
著者 |
Spracklin G, Fields B, Wan G, Becker D, Wallig A, Shukla A, Kennedy S.
|
タイトル |
The RNAi Inheritance Machinery of Caenorhabditis elegans.
|
ジャーナル |
Genetics
|
Abstract |
Gene silencing mediated by dsRNA (RNAi) can persist for multiple generations in Caenorhabditis elegans (termed RNAi inheritance). Here we describe the results of a forward genetic screen in C. elegans that has identified six factors required for RNAi inheritance: GLH-1/VASA, PUP-1/CDE-1, MORC-1, SET-32, and two novel nematode-specific factors that we term here (heritable RNAi defective) HRDE-2 and HRDE-4 The new RNAi inheritance factors exhibit mortal germline (Mrt) phenotypes, which we show is likely caused by epigenetic deregulation in germ cells. We also show that HRDE-2 contributes to RNAi inheritance by facilitating the binding of small RNAs to the inheritance Argonaute (Ago) HRDE-1 Together, our results identify additional components of the RNAi inheritance machinery whose conservation provides insights into the molecular mechanism of RNAi inheritance, further our understanding of how the RNAi inheritance machinery promotes germline immortality, and show that HRDE-2 couples the inheritance Ago HRDE-1 with the small RNAs it needs to direct RNAi inheritance and germline immortality.
|
巻・号 |
206(3)
|
ページ |
1403-1416
|
公開日 |
2017-7-1
|
DOI |
10.1534/genetics.116.198812
|
PII |
genetics.116.198812
|
PMID |
28533440
|
PMC |
PMC5500139
|
MeSH |
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Epigenesis, Genetic
Gene Silencing*
Phenotype
RNA, Small Interfering / genetics*
|
IF |
4.015
|
引用数 |
35
|
リソース情報 |
線虫 |
tm1200
tm1358
tm6048 |