論文 - 詳細
RRC ID | 47328 |
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著者 | Carr D, Sanchez-Alvarez L, Imai JH, Slatculescu C, Noblett N, Mao L, Beese L, Colavita A. |
タイトル | A Farnesyltransferase Acts to Inhibit Ectopic Neurite Formation in C. elegans. |
ジャーナル | PLoS One |
Abstract |
Genetic pathways that regulate nascent neurite formation play a critical role in neuronal morphogenesis. The core planar cell polarity components VANG-1/Van Gogh and PRKL-1/Prickle are involved in blocking inappropriate neurite formation in a subset of motor neurons in C. elegans. A genetic screen for mutants that display supernumerary neurites was performed to identify additional factors involved in this process. This screen identified mutations in fntb-1, the β subunit of farnesyltransferase. We show that fntb-1 is expressed in neurons and acts cell-autonomously to regulate neurite formation. Prickle proteins are known to be post-translationally modified by farnesylation at their C-terminal CAAX motifs. We show that PRKL-1 can be recruited to the plasma membrane in both a CAAX-dependent and CAAX-independent manner but that PRKL-1 can only inhibit neurite formation in a CAAX-dependent manner. |
巻・号 | 11(6) |
ページ | e0157537 |
公開日 | 2016-1-1 |
DOI | 10.1371/journal.pone.0157537 |
PII | PONE-D-16-03536 |
PMID | 27300162 |
PMC | PMC4907426 |
MeSH | Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Farnesyltranstransferase / analysis Farnesyltranstransferase / genetics Farnesyltranstransferase / metabolism* Intracellular Signaling Peptides and Proteins / analysis Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Models, Molecular Mutation Neurites / physiology* Prenylation Protein Processing, Post-Translational Protein Subunits / analysis Protein Subunits / genetics Protein Subunits / metabolism |
IF | 2.74 |
引用数 | 1 |
WOS 分野 | MULTIDISCIPLINARY SCIENCES |
リソース情報 | |
線虫 | vang-1(tm1422) |