論文 - 詳細
RRC ID | 47731 |
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著者 | Pelisch F, Tammsalu T, Wang B, Jaffray EG, Gartner A, Hay RT. |
タイトル | A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis. |
ジャーナル | Mol Cell |
Abstract |
During Caenorhabditis elegans oocyte meiosis, a multi-protein ring complex (RC) localized between homologous chromosomes, promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. We show that SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC, and proteomic analysis identified KLP-19 as a SUMO substrate in vivo. In vitro analysis revealed that KLP-19 is efficiently sumoylated in a GEI-17-dependent manner, while GEI-17 undergoes extensive auto-sumoylation. GEI-17 and another RC component, the kinase BUB-1, contain functional SUMO interaction motifs (SIMs), allowing them to recruit SUMO modified proteins, including KLP-19, into the RC. Thus, dynamic SUMO modification and the presence of SIMs in RC components generate a SUMO-SIM network that facilitates assembly of the RC. Our results highlight the importance of SUMO-SIM networks in regulating the assembly of dynamic protein complexes. |
巻・号 | 65(1) |
ページ | 66-77 |
公開日 | 2017-1-5 |
DOI | 10.1016/j.molcel.2016.11.001 |
PII | S1097-2765(16)30709-2 |
PMID | 27939944 |
PMC | PMC5222697 |
MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Positioning* Chromosome Segregation* Female Genotype Kinesins / genetics Kinesins / metabolism* Ligases / genetics Ligases / metabolism* Meiosis* Oocytes / metabolism* Phenotype Protein Binding Protein Interaction Domains and Motifs Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Signal Transduction Sumoylation* Time Factors Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism* |
IF | 15.584 |
引用数 | 22 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
線虫 | tm2723 & tm2610 alleles |