RRC ID 47731
著者 Pelisch F, Tammsalu T, Wang B, Jaffray EG, Gartner A, Hay RT.
タイトル A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis.
ジャーナル Mol Cell
Abstract During Caenorhabditis elegans oocyte meiosis, a multi-protein ring complex (RC) localized between homologous chromosomes, promotes chromosome congression through the action of the chromokinesin KLP-19. While some RC components are known, the mechanism of RC assembly has remained obscure. We show that SUMO E3 ligase GEI-17/PIAS is required for KLP-19 recruitment to the RC, and proteomic analysis identified KLP-19 as a SUMO substrate in vivo. In vitro analysis revealed that KLP-19 is efficiently sumoylated in a GEI-17-dependent manner, while GEI-17 undergoes extensive auto-sumoylation. GEI-17 and another RC component, the kinase BUB-1, contain functional SUMO interaction motifs (SIMs), allowing them to recruit SUMO modified proteins, including KLP-19, into the RC. Thus, dynamic SUMO modification and the presence of SIMs in RC components generate a SUMO-SIM network that facilitates assembly of the RC. Our results highlight the importance of SUMO-SIM networks in regulating the assembly of dynamic protein complexes.
巻・号 65(1)
ページ 66-77
公開日 2017-1-5
DOI 10.1016/j.molcel.2016.11.001
PII S1097-2765(16)30709-2
PMID 27939944
PMC PMC5222697
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Positioning* Chromosome Segregation* Female Genotype Kinesins / genetics Kinesins / metabolism* Ligases / genetics Ligases / metabolism* Meiosis* Oocytes / metabolism* Phenotype Protein Binding Protein Interaction Domains and Motifs Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism Signal Transduction Sumoylation* Time Factors Ubiquitin-Protein Ligases / genetics Ubiquitin-Protein Ligases / metabolism*
IF 15.584
引用数 22
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm2723 & tm2610 alleles