RRC ID 49517
著者 Kondo S, Vedanayagam J, Mohammed J, Eizadshenass S, Kan L, Pang N, Aradhya R, Siepel A, Steinhauer J, Lai EC.
タイトル New genes often acquire male-specific functions but rarely become essential in Drosophila.
ジャーナル Genes Dev
Abstract Relatively little is known about the in vivo functions of newly emerging genes, especially in metazoans. Although prior RNAi studies reported prevalent lethality among young gene knockdowns, our phylogenomic analyses reveal that young Drosophila genes are frequently restricted to the nonessential male reproductive system. We performed large-scale CRISPR/Cas9 mutagenesis of "conserved, essential" and "young, RNAi-lethal" genes and broadly confirmed the lethality of the former but the viability of the latter. Nevertheless, certain young gene mutants exhibit defective spermatogenesis and/or male sterility. Moreover, we detected widespread signatures of positive selection on young male-biased genes. Thus, young genes have a preferential impact on male reproductive system function.
巻・号 31(18)
ページ 1841-1846
公開日 2017-9-15
DOI 10.1101/gad.303131.117
PII 31/18/1841
PMID 29051389
PMC PMC5695085
MeSH Animals CRISPR-Cas Systems / genetics Drosophila melanogaster / genetics* Evolution, Molecular Fertility / genetics* Frameshift Mutation Gene Expression Gene Expression Profiling Gene Knockdown Techniques Genes, Essential / physiology* Genes, Insect / physiology* Genes, Lethal / physiology Infertility, Male / genetics Male Phylogeny RNA Interference Reproduction / genetics* Spermatogenesis / genetics Testis / anatomy & histology Testis / metabolism
IF 9.527
引用数 11
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