RRC ID 48843
著者 Ikeda K, Nagata S, Okitsu T, Takeuchi S.
タイトル Cell fiber-based three-dimensional culture system for highly efficient expansion of human induced pluripotent stem cells.
ジャーナル Sci Rep
Abstract Human pluripotent stem cells are a potentially powerful cellular resource for application in regenerative medicine. Because such applications require large numbers of human pluripotent stem cell-derived cells, a scalable culture system of human pluripotent stem cell needs to be developed. Several suspension culture systems for human pluripotent stem cell expansion exist; however, it is difficult to control the thickness of cell aggregations in these systems, leading to increased cell death likely caused by limited diffusion of gases and nutrients into the aggregations. Here, we describe a scalable culture system using the cell fiber technology for the expansion of human induced pluripotent stem (iPS) cells. The cells were encapsulated and cultured within the core region of core-shell hydrogel microfibers, resulting in the formation of rod-shaped or fiber-shaped cell aggregations with sustained thickness and high viability. By encapsulating the cells with type I collagen, we demonstrated a long-term culture of the cells by serial passaging at a high expansion rate (14-fold in four days) while retaining its pluripotency. Therefore, our culture system could be used for large-scale expansion of human pluripotent stem cells for use in regenerative medicine.
巻・号 7(1)
ページ 2850
公開日 2017-6-6
DOI 10.1038/s41598-017-03246-2
PII 10.1038/s41598-017-03246-2
PMID 28588295
PMC PMC5460280
MeSH Animals Cell Count Cell Culture Techniques* / methods Cell Differentiation Cell Proliferation Cells, Cultured Extracellular Matrix Humans Induced Pluripotent Stem Cells / cytology* Mice
IF 3.998
引用数 10
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ヒト・動物細胞 409B2(HPS0076)