RRC ID 37517
著者 Kobayashi H, Nobeyama Y, Nakagawa H.
タイトル Tumor-suppressive effects of natural-type interferon-β through CXCL10 in melanoma.
ジャーナル Biochem Biophys Res Commun
Abstract INTRODUCTION:Type 1 interferon is in widespread use as adjuvant therapy to inhibit melanoma progression. Considering the tumor-suppressive effects of local administration of interferon-β (IFN-β) on lymphatic metastasis, the present study was conducted to identify melanoma-suppressive molecules that are up-regulated by IFN-β treatment of lymphatic endothelial cells.
MATERIALS AND METHODS:Lymphatic endothelial cells, fibroblasts, and melanoma cells were treated with natural-type IFN-β, and melanoma cells were treated with CXCL10. Genome-wide oligonucleotide microarray analysis was performed using lymphatic endothelial cells with or without IFN-β treatment. Quantitative real-time reverse transcription-PCR and an enzyme-linked immunosorbent assay were performed to examine CXCL10 expression. A proliferation assay was performed to examine the effects of IFN-β and CXCL10 in melanoma cells.
RESULTS:Genome-wide microarray analyses detected CXCL10 as a gene encoding a secretory protein that was up-regulated by IFN-β in lymphatic endothelial cells. IFN-β treatment significantly induced CXCL10 in dermal lymphatic endothelial cells and melanoma cells that are highly sensitive to IFN-β. CXCL10 reduced melanoma cell proliferation in IFN-β-sensitive cells as well as resistant cells. Melanoma cells in which CXCL10 was knocked down were sensitive to IFN-β. CXCR3-B, which encodes the CXCL10 receptor, was up-regulated in melanoma cells with high sensitivity to IFN-β and down-regulated in melanoma cells with medium to low sensitivity.
CONCLUSIONS:Our data suggest that IFN-β suppresses proliferation and metastasis from the local lymphatic system and melanoma cells via CXCL10. Down-regulation of CXCR3-B by IFN-β may be associated with resistance to IFN-β.
巻・号 464(2)
ページ 416-21
公開日 2015-8-21
DOI 10.1016/j.bbrc.2015.06.122
PII S0006-291X(15)30176-5
PMID 26102027
MeSH Cell Line, Tumor Chemokine CXCL10 / genetics Chemokine CXCL10 / physiology* Down-Regulation Gene Knockdown Techniques Humans Interferon-beta / physiology* Melanoma / pathology* Receptors, CXCR3 / physiology Up-Regulation
IF 2.985
引用数 5
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 NB1RGB(RCB0222) G-361(RCB0991)