RRC ID |
43897
|
著者 |
Ohgidani M, Ichihara H, Goto K, Matsumoto Y, Ueoka R.
|
タイトル |
Anticancer effects of residual powder from Barley-Shochu distillation remnants against the orthotopic xenograft mouse models of hepatocellular carcinoma in vivo.
|
ジャーナル |
Biol Pharm Bull
|
Abstract |
Barely-Shochu is a traditional Japanese liquor distilled from fermented barley with Saccharomyces cerevisiae. Barely-Shochu distillation remnants (SDR) are by-products in the manufacturing process of barley-Shochu. We have already reported on valuable powder from Shochu distillation remnants (PSDR) including antioxidative compounds such as polyphenols. In this study, we investigated the therapeutic effects of barely-PSDR against orthotopic xenograft mouse models of hepatocellular carcinoma (HCC) in vivo. We constructed a mouse model of HCC by orthotopical inoculation of HepG2 cells into the liver of SCID mice. Barely-PSDR (2250 mg/kg) was orally treated once each day for 21 d after the inoculation of HepG2 cells. The livers were removed from anaesthetized mice after the treatment with barely-PSDR and fixed in formalin. The liver sections were analyzed by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) methods. Remarkably high reduction of tumorigenesis was obtained in the mouse models of HCC after the oral administration of barely-PSDR in vivo. Induction of apoptosis in the liver section on the mouse models treated with barely-PSDR was observed. Furthermore, prolonged survival was obtained. Thus, therapeutic effects of barely-PSDR without side effects on the orthotopic xenograft mouse models were revealed for the first time.
|
巻・号 |
35(6)
|
ページ |
984-7
|
公開日 |
2012-1-1
|
DOI |
10.1248/bpb.35.984
|
PII |
DN/JST.JSTAGE/bpb/35.984
|
PMID |
22687544
|
MeSH |
Animals
Antineoplastic Agents, Phytogenic / pharmacology
Antineoplastic Agents, Phytogenic / therapeutic use*
Apoptosis / drug effects
Carcinoma, Hepatocellular / drug therapy*
Carcinoma, Hepatocellular / pathology
Distillation
Female
Hep G2 Cells
Hordeum*
Humans
Liver Neoplasms / drug therapy*
Liver Neoplasms / pathology
Mice
Mice, SCID
Plant Extracts / pharmacology
Plant Extracts / therapeutic use*
Tumor Burden / drug effects
Xenograft Model Antitumor Assays
|
IF |
1.863
|
引用数 |
6
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
リソース情報 |
ヒト・動物細胞 |
RERF-LC-AI(RCB0444)
HuH-7
Hep G2 |