RRC ID 29713
著者 Fujita N, Nagata Y, Nishiuchi T, Sato M, Iwami M, Kiya T.
タイトル Visualization of neural activity in insect brains using a conserved immediate early gene, Hr38.
ジャーナル Curr Biol
Abstract Many insects exhibit stereotypic instinctive behavior [1-3], but the underlying neural mechanisms are not well understood due to difficulties in detecting brain activity in freely moving animals. Immediate early genes (IEGs), such as c-fos, whose expression is transiently and rapidly upregulated upon neural activity, are powerful tools for detecting behavior-related neural activity in vertebrates [4, 5]. In insects, however, this powerful approach has not been realized because no conserved IEGs have been identified. Here, we identified Hr38 as a novel IEG that is transiently expressed in the male silkmoth Bombyx mori by female odor stimulation. Using Hr38 expression as an indicator of neural activity, we mapped comprehensive activity patterns of the silkmoth brain in response to female sex pheromones. We found that Hr38 can also be used as a neural activity marker in the fly Drosophila melanogaster. Using Hr38, we constructed a neural activity map of the fly brain that partially overlaps with fruitless (fru)-expressing neurons in response to female stimulation. These findings indicate that Hr38 is a novel and conserved insect neural activity marker gene that will be useful for a wide variety of neuroethologic studies.
巻・号 23(20)
ページ 2063-70
公開日 2013-10-21
DOI 10.1016/j.cub.2013.08.051
PII S0960-9822(13)01057-9
PMID 24120640
MeSH Animals Bombyx / genetics Bombyx / physiology* Brain / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Female Genes, Immediate-Early* Insect Proteins / genetics* Insect Proteins / metabolism Male Molecular Sequence Data Neurons / metabolism Pheromones / metabolism Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / metabolism Sequence Analysis, DNA Sex Attractants / metabolism
IF 9.601
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
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