RRC ID 43090
著者 Zhang Y, Li Y, Shibahara S, Takahashi K.
タイトル Synergistic activation of the human adrenomedullin gene promoter by Sp1 and AP-2alpha.
ジャーナル Peptides
Abstract Adrenomedullin (AM) is a potent vasodilator peptide, which is ubiquitously expressed and has various biological actions, such as proliferative action and anti-oxidative stress action. AM expression is induced by various stresses, such as hypoxia and inflammatory cytokines, and during cell differentiation. The human AM gene promoter region (-70/-29) contains binding sites for stimulatory protein 1 (Sp1) and activator protein-2alpha (AP-2alpha), and has been shown to be important for the AM gene expression during cell differentiation to macrophages or adipocytes. We here show that Sp1 and AP-2alpha synergistically activate the AM gene promoter. Co-transfection of the reporter plasmid containing the AM promoter region (-103/-29) with Sp1 and AP-2alpha expression plasmids showed that Sp1 and AP-2alpha synergistically increased the promoter activity in HeLa cells. Sp1 or AP-2alpha alone caused only small increases in the promoter activity. EMSA showed that Sp1 bound to the promoter region (-70/-29), whereas AP-2alpha bound to a more upstream promoter region (-103/-71). Thus, the synergistic activation of the human AM gene promoter by Sp1 and AP-2alpha may be mediated by the binding of Sp1 to the promoter region (-70/-29) and the interaction with AP-2alpha, which binds to the promoter region (-103/-71).
巻・号 29(3)
ページ 465-72
公開日 2008-3-1
DOI 10.1016/j.peptides.2007.07.015
PII S0196-9781(07)00236-7
PMID 17719138
MeSH Adrenomedullin / genetics* Binding Sites / genetics Electrophoretic Mobility Shift Assay Gene Expression Regulation HeLa Cells Humans Promoter Regions, Genetic / genetics* Protein Binding Sp1 Transcription Factor / genetics Sp1 Transcription Factor / metabolism* Transcription Factor AP-2 / genetics Transcription Factor AP-2 / metabolism* Transfection
IF 2.843
引用数 5
WOS 分野 PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 HeLa(RCB0007)